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Whitton, S.R.; McKenzie, E.H.C.; Hyde, K.D. 2012: Fungi Associated with Pandanaceae. Hyde, K.D. (ed). Fungal Diversity Research Series. 21.

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Whitton, S.R.; McKenzie, E.H.C.; Hyde, K.D. 2012: Fungi Associated with Pandanaceae. Hyde, K.D. (ed). Fungal Diversity Research Series. 21.
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COMMENTS: Both Berkleasmium nigroapicale Bussaban, Lumyong, P. Lumyong, McKenzie & K.D. Hyde and B. monilicellulare (see below) have conidia with a black, opaque apex. However, the conidia of B. nigroapicale are smaller (24–27 × 12.5–15 m) with fewer cells. The conidia of B. monilicellulare are slightly smaller (33–45 × 20–27 m) than those of B. atroapicale and approximately three quarters of the conidia are black and opaque rather than the top one third in B. atroapicale. In addition, B. atroapicale is the only species of Berkleasmium that has its conidia borne on short, globose cells, all other species produce more or less cylindrical (sometimes swollen), often septate conidiophores (Moore, 1958, 1959; Ellis, 1971, 1976; Sharma, 1980; Matsushima, 1981, 1983, 1987).
COMMENTS: Coronospora novae-zelandiae is characterised by 0–4-septate, 18–37 × 6–9 m conidia that have small, rounded projections at the apex (Matsushima, 1985). All morphological characters and dimensions of the current specimen agree with the original description. C. novae-zelandiae, described from a single specimen isolated from dead leaves of Rhopalostylis sapida, is the commonest hyphomycete found on dead leaves of Freycinetia banksii (McKenzie and Hyde, 1997).
NOTES: Crocicreas novae-zelandiae is characterised by broadly elliptical, aseptate ascospores, and the very small size of the short stipitate apothecia. Only a few species of Crocicreas have aseptate, broadly elliptical ascospores. Crocicreas calathicola (Rehm) S.E. Carp. has similarly shaped ascospores but are somewhat clavate and smaller (5–9 × 2–3 µm); the asci are also smaller (55–63 × 4–5 µm) and the apothecia are much larger (1–4 mm diam.) (Carpenter, 1981). The apothecia of C. eschscholtziae (W. Phillips & Harkn.) S.E. Carp. are larger (1–1.5 mm diam.) and dark brown, the asci are smaller (50–65 × 4–6 µm) than those in C. novae-zelandiae, as are the elliptical ascospores (7–12 × 2–3 µm). The dark brown colour and swollen apical cells in the hyphae of the ectal excipulum (Carpenter, 1981) is a character not present in the current specimens. C. espeletarum S.E. Carp. has smaller asci (43–52 × 7–8 µm), smaller ascospores (7–11 × 3.5–4 µm) and larger apothecia (500–750 µm diam.) that have distinct, triangular marginal teeth (Carpenter, 1981).
COMMENTS: Dictyosporium acroinflatum is characterised by pale brown conidia with tapering rows of cells that are often partly or wholly uncemented and overlap each other irregularly. The septa are constricted and cell rows are frequently terminated by hyaline, globose, swollen apices.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Wairou-Cosseys Track, on decaying leaves of Freycinetia banksii, 9 May 1996, S.R. Whitton HKU(M)12748 (IFRD209-007); North Island, Auckland Region, Waitakere Ranges, Spraggs Track, on decaying leaves of F. banksii, 23 May 1996, S.R. Whitton HKU(M)12746 (IFRD209-005); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 2 June 1996, S.R. Whitton HKU(M)12749 (IFRD8993, holotype); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 1 July 1996, S.R. Whitton HKU(M)12747 (IFRD209-006).
NOTES: These specimens are referred to Hyalorbilia inflatula based primarily on ascospore shape and dimensions, ascus dimensions, the fact that the paraphyses exceed the asci and form an apithelium and the hyaline apothecia. However, H. inflatula is reported to have broad paraphyses which are not greatly enlarged at the apex, and has asci which typically do not have tapered and/or branched bases (Spooner, 1987). In the present specimens the paraphyses are narrow with swollen apices, and the asci have tapered bases. This is considered to represent natural variation. The single specimen from Hong Kong HKU(M)12838 had somewhat longer asci (26–33 × 3–3.5 µm) than has been reported previously for H. inflatula (24–28 × 4 µm ) (Spooner, 1987), but is still considered to be H. inflatula based on its ascospore characters and dimensions. MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Wairou-Cosseys Track, on decaying leaves of Freycinetia banksii, 9 May 1996, S.R. Whitton HKU(M)4957 (IFRD); North Island, Auckland Region, Waitakere Ranges, Fairy Falls Track, on decaying leaves of F. banksii, 23 May 1996, S.R. Whitton HKU(M)4958 (IFRD213-011); North Island, Wellington Region, Paraparaumu, Nikau Reserve, on decaying leaf bases of F. banksii, 6 May 1997, S.R. Whitton HKU(M)5084 (IFRD212-016); North Island, Wellington Region, Wainuimata Valley, in forest surrounding Wainuiomata Reservoir, on decaying leaves of F. banksii, 6 May 1997, S.R. Whitton HKU(M)12739 (IFRD).
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Wairou-Cosseys Track, on decaying leaves of Freycinetia banksii, 9 May 1996, S.R. Whitton HKU(M)5051 (IFRD210-019); North Island, Auckland Region, Hunua Ranges, Workman Track, decaying leaves of F. banksii, 8 May 1996, S.R. Whitton HKU(M)5049 (IFRD210-017). NOTES: Hydropisphaera arenuloides is characterised by globose ascomata that are sessile and more or less glabrous. Asci are 60–100 × 9–18 µm and ascospores are 13–24 × 3.5–5 µm, fusiform, 1-septate, not constricted at the septum and striate (Samuels, 1976a). Based on ascomatal morphology and dimensions, these specimens are best treated as H. arenuloides. No taxonomically significant differences between the current specimens and those reported by Samuels (1976a) were observed.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of Freycinetia banksii, 1 May 1997, S.R. Whitton HKU(M)5063 (IFRD212-002). NOTES: Hydropisphaera erubescens is characterised by sessile, orange to orange-brown, globose ascomata and 55–84 × 9–15 µm asci. The ascospores are 17–29 × 3.5–5 µm, 3–4-septate, smooth, slightly constricted at the septa and hyaline (Samuels, 1978). Specimens HKU(M)5063, HKU(M)12800 and HKU(M)14049 are faintly ornamented and none of the collections have spores with constricted septa. These differences aside, ascomata morphology and ascospores size, septation and shape all agree with the description given by Samuels (1978) for H. erubescens. The faint ascospore ornamentation is thought to represent natural variation amongst specimens.
COMMENTS: Based on the successive, annellidic proliferation of the conidiophores, and conidia that remain attached to the side of the conidiophore following conidiophore proliferation, the current specimen is best treated in Ityorhoptrum. The other described species, I. verruculosum has larger (15–19 × 5.5–7.5 m), 2-celled, obovoid to clavate conidia (Hughes, 1979b; Rao and de Hoog, 1986).
MATERIAL EXAMINED: NEW ZEALAND, North Island, Manawatu Region, Manawatu Gorge Scenic Reserve, on decaying leaves of Freycinetia banksii, 30 April 1997, S.R. Whitton HKU(M)5080 (IFRD212-013); North Island, Auckland Region, Hunua Ranges, Wairoa-Cosseys track, on decaying leaves of F. banksii, 23 May 1996, S.R. Whitton HKU(M)4971 (IFRD215-016); North Island, Wellington Region, in the forest alongside Akatarawa Hill Road, on decaying leaves of F. banksii, 1 May 1997, S.R. Whitton HKU(M)14036 (IFRD154-007); North Island, Wellington Region, Wainuiomata Valley, in the forest surrounding Wainuiomata Reservoir, on decaying leaves of F. banksii, 6 May 1997, S.R. Whitton HKU(M)14037 (IFRD154-008).
NOTES: Four species of Lachnum have stipitate, white apothecia, with small aseptate, cylindric ascospores and lanceolate paraphyses: L. candidum (Rodway) Spooner, L. curtipulum Spooner, L. nudipes and L. virgineum (Batsch) P. Karst. All four species are similar in overall morphology, differing mainly in regard to ascospore shape and dimensions, and paraphyses width and length (Spooner, 1987). In L. nudipes the ascospores are narrowly fusoid, aseptate and 11–15 × 1.5–2 µm, and the paraphyses are broadly lanceolate (4.5–7 µm wide) and 20–30 µm longer than the asci (Spooner, 1987). The ascospores dimensions in L. curtipilum are 5.5–8 × 1.5–2 µm, which is almost identical to that found in the current specimens, whilst the ascospores are 7–10.5 × 1.5–2 µm in L. virgineum, and 7–11.5 × 2–2.5 µm in L. candidum (Spooner, 1987). Even though the ascospores of L. curtipilum are reported to be clavate-ellipsoid (Spooner, 1987), and the spores of the present specimens are all short cylindrical to ellipsoid, the dimensions fit better with L. curtipilum than they do for L. virgineum.
NOTES: As noted by Spooner (1987) Lachnum hyalopus is probably closely related to L. apalum, which has ascospores and apothecia of similar morphology, differing primarily due to its wider paraphyses (2.5–5 µm) and narrower ascospores (34–42 × 1.2–1.5 µm). As this specimen has narrowly lanceolate paraphyses, it is best treated in L. hyalopus. The only differences between this specimen and that reported by Spooner (1987) are slightly shorter asci (85–95 × 7.5–8.5 µm) and slightly narrower ascospores (1.5–2 µm, L. hyalopus); these differences are concluded to represent natural variation.
NOTES: The current specimen is characterized primarily by its unusual ascospore morphology. Few species of Lachnum have short cylindrical, 1-septate, slightly curved ascospores. Lachnum nudipes (Fuckel) Nannf. is similar in morphology to the current specimen, especially in respect to dimensions and shape of ascospores. The ascospores of L. nudipes are aseptate, narrowly fusoid and 11–15 × 1.5–2 µm; the paraphyses are broadly lanceolate (4.5–7 µm wide) and exceed the asci by 20–30 µm. As the specimen is scanty and in poor condition, specific recognition at this time is inappropriate
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Waharau Regional Park, on decaying leaves of Freycinetia banksii, 7 May 1996, S.R. Whitton HKU(M)4952 (IFRD213-009); HKU(M)4968 (IFRD215-014) North Island, Rotorua Region, in the forest surrounding Lake Okatina, on decaying leaves of F. banksii, 25 May 1996, S.R. Whitton HKU(M)4953 (IFRD213-010); North Island, Manawatu Region, Manawatu Gorge Scenic Reserve, on decaying leaves of F. banksii, 30 May 1997, S.R. Whitton HKU(M)5079 (IFRD212-011); North Island, Wellington Region, in the forest beside Akatarawa Hill Road, on decaying leaves of F. banksii, 1 May 1997, S.R. Whitton HKU(M)14038 (IFRD154-009); North Island, Wellington Region, Wainuiomata Valley, in forest surrounding Wainuiomata Reservoir, on decaying leaves of F. banksii, 6 May 1997, S.R. Whitton HKU(M)14039 (IFRD154-010); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 6 May 1997, S.R. Whitton HKU(M)5085 (IFRD212-017).
NOTES: Lasiobelonium variegatum is characterized by 1-septate, fusoid ascospores (10–13.5 × 2–2.5 µm), cylindric-clavate asci (59–75 × 5–5.5 µm), and hairs (120–170 × 3–4.5 µm), which have slightly thickened walls, which are pale yellow, and are often adorned with scattered, irregular granules especially towards the apex. The ascal apical pore is stained blue in Melzer’s reagent, and in situ the apothecium is short stipitate, and is a brown-yellow colour (Spooner, 1987). Apart from some small differences in dimensions all specimens agree with the description given by Spooner (1987); the differences are concluded to be within the bounds of natural variation.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of Freycinetia banksii, 1 May 1997, S.R. Whitton HKU(M)5065 (IFRD212-004). NOTES: Lasionectria sylvana is characterised by sessile, solitary or clustered in small groups, pale to orange-brown ascomata with an irregular fringe of triangular, fasciculate hairs surrounding the ostiolar region. Asci 30–85 × 6–11 µm and the ascospores are 9–17 × 2.5–5 µm, hyaline, 1-septate, not or slightly constricted at the septum and smooth to striate (Samuels, 1976a). Based on the irregular arrangement of fasciculate hairs, ascomatal pigmentation, and ascospore morphology and dimensions, these specimens are best treated as L. sylvana. The ascospores of HKU(M)5045 have coarse striations. No taxonomically significant differences between the present specimens and those reported by Samuels (1976a) were observed.
NOTES: Nectriopsis anthostomellicola is characterised by being associated with an unidentified and immersed species of Anthostomella growing on Freycinetia banksii. The ascomata are small, pale orange, globose, and composed of a narrow layer of simple, thin-walled cells. A small group of ascomata encircles the host ostiole and is always surrounded by hyphal tissue, which often forms fasciculate hairs. Of the 43 species of Nectriopsis treated by Samuels (1988a) only N. squamulosa (Ellis) Samuels and N. epimyces Samuels are associated with immersed ascomycetes. In N. squamulosa the ascomata are similar to N. anthostomellicola in being thin-walled, smooth, yellow to yellow-orange and surrounded by hyphae. However, the hyphae do not form erect, fasciculate hairs and the ascospores are slightly smaller (6–11 × 1.5–3 µm) and smooth (Samuels, 1988a). In N. epimyces the perithecia are also surrounded by white mycelium, and have similar sized asci (27–50 × 5.5–8.5 µm) and ascospores (10–16.2 × 2–3.5 µm). However, the ascospores are smooth, the hyphae surrounding the perithecia do not form erect, fasciculate hairs, the apex of the perithecia is formed of hyphal elements that merge with the periphyses and the perithecial walls are somewhat thickened (Samuels, 1988a).
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Wairoa-Cosseys Track, on decaying leaves of Freycinetia banksii, 9 May 1996, S.R. Whitton HKU(M)12855 (IFRD218-006); North Island, Auckland Region, Waitakere Ranges, Spraggs Track, on decaying leaves of F. banksii, 23 May 1996, S.R. Whitton HKU(M)12851 (IFRD218-003); HKU(M)12852 (IFRD218-006); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 2 June 1996, S.R. Whitton HKU(M)12856 (IFRD218-007).
NOTES: Niesslia kapitiana is characterised by the small size and hooked nature of the setae and by the scattered arrangement of the setae over the ascomatal surface. The ascospores are also unusual in being spinulose, 0–1 septate, 13–21.5 × 4–5.5 µm and surrounded by a gelatinous sheath. No reported species of Niesslia have ascospores of this size and morphology. Niesslia vaginata (current material) has ascospores that are finely verrucose, and surrounded by a gelatinous sheath. However, in that species the ascospores are 9.5–13 × 2–3 µm, and the setae are straight and 30–64 µm long and 8.5–16 µm wide at the base. MATERIAL EXAMINED: NEW ZEALAND, North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of Freycinetia banksii, 1 May 1997, S.R. Whitton HKU(M)12879 (IFRD, holotype).
COMMENTS: Niptera purpurascens is characterized by large asci, long, multiseptate, thick-walled ascospores, and unique purple reaction of the ascospores in Melzer’s reagent. No species of Niptera are known to have multiseptate ascospores of this length which turn purple in Melzer’s reagent. Niptera excelsior (P. Karst.) Dennis has similarities to the current specimens in having 3-septate, elongate ascospores. However, the asci (up to 130 × 10 µm) and ascospores (50–80 × 3–4 µm) are smaller, and no indication of an amyloid reaction was noted (Dennis, 1972, 1981). The current species is placed in Niptera based primarily on the elongate, multiseptate ascospores and the ectal excipular morphology.
NOTES: Orbilia juruensis is reported to have apothecia 2–6 mm in diameter, asci 39–47 × 3.5–4 µm, and fusoid ascospores 5.5–8 × 1.5 µm (Spooner, 1987). Apart from the apothecial diameter, this specimen agrees with the description for O. juruensis (Spooner, 1987). The difference in apothecial diameter is considered to be consistent with natural variation. MATERIAL EXAMINED: NEW ZEALAND, North Island, Northern Hawke Bay, White Pine Bush Reserve, on decaying leaves of Freycinetia banksii, 30 April 1997, S.R. Whitton HKU(M)5078 (IFRD212-010).
MATERIAL EXAMINED: NEW ZEALAND, North Island, Manawatu Region, Manawatu Gorge Scenic Reserve, on decaying leaves of Freycinetia banksii, 30 April 1997, S.R. Whitton HKU(M)5081 (IFRD212-013). NOTES: Orbilia tenuissima is characterised by light yellow apothecia, and cylindrical ascospores that are 8–10 × 0.5 µm and slightly curved. The asci of O. tenuissima are 20 × 3 µm and apically truncate (Dennis, 1970). The morphological characters and microscopic dimensions of this specimen agree with those for O. tenuissima.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Manawatu Region, Manawatu Scenic Reserve, on decaying leaves of Freycinetia banksii, 30 Apr. 1997, S.R. Whitton HKU(M)12860 (IFRD8999, holotype). NOTES: Ornatispora novae-zelandiae is distinguished from all other species by the Stachybotrys freycinetiae anamorph. It has much larger ascospores than Ornatispora nepalensis.
NEW ZEALAND, North Island, Manawatu Region, Manawatu Gorge Scenic Reserve, on decaying leaves of Freycinetia banksii, 30 April 1997, S.R. Whitton HKU(M)14062 (IFRD151-013); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 1 May 1997, S.R. Whitton HKU(M)14063 (IFRD151-014).
NOTES: Peristomialis cellularis is characterised by solitary, lightly pigmented, more or less globose ascomata, which have simple or branched appendages composed of irregularly intertwined hyphae. Based on the morphology of the ascomatal wall and appendage tissues this species is best treated as a member of Peristomialis, close to P. parilis (H. Sydow) Samuels and P. paraparilis Samuels (Samuels, 1988a). In P. paraparilis and P. parilis the ascomata are similar to P. cellularis in being made of intertwined hyphae and having appendages also composed of intertwined hyphae. However, in P. paraparilis the asci are 63–80 × 12.5–18 µm and the ascospores are 1-septate, coarsely striate and 19.5–25.5 × 4–5 µm. Peristomialis parilis differs in having a narrow ostiolar opening and well developed, longer, fasciculate appendages, which are typically produced around the ostiolar region but can be scattered irregularly over the entire perithecial surface. These appendages can also be irregularly branched. The appendages of P. parilis are described as “short, fasciculate, triangular hairs or wart-like processes” and are produced around the ostiolar disc (Samuels, 1988a). As the asci and ascospores of P. parilis are very similar to the current specimens these two taxa may prove to be conspecific, but based on the narrow ostiolar opening, the well developed fasciculate appendages and their development over the entire perithecial surface, P. cellularis is introduced as new.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Workman Track, on decaying leaves of Freycinetia banksii, 8 May 1996, S.R. Whitton HKU(M)12701 (IFRD8992, holotype); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 2 June 1996, S.R. Whitton HKU(M)5069 (IFRD212-005); North Island, Kapiti Coast, north of Paraparaumu, Nikau Reserve, on decaying leaves of F. banksii, 1 May 1997, S.R. Whitton HKU(M)5061 (IFRD210-025); HKU(M)5062 (IFRD210-025);; North Island, Wellington Region, Wainuiomata Valley, in the forest surrounding Wainuiomata Reservoir, on decaying leaves of F. banksii, 6 May 1997, S.R. Whitton HKU(M)12885 (IFRD220-005).
NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Workman Track, on decaying leaves of Freycinetia banksii, 8 July 1996, S.R. Whitton HKU(M)14103 (IFRD158-015); North Island, Auckland Region, Hunua Ranges, Wairoa-Cosseys, on decaying leaves of F. banksii, 9 July 1996, S.R. Whitton HKU(M)14102 (IFRD158-014); North Island, Rotorua Region, in forest surrounding Lake Okatina, on decaying leaves of F. banksii, 25 July 1996, S.R. Whitton HKU(M)14104 (IFRD158-016).
MATERIAL EXAMINED: NEW ZEALAND, North Island, Wellington Region, Wainuiomata Valley, in forest surrounding Wainuiomata Reservoir, on decaying stems and aerial roots of Freycinetia banksii, 7 May 1997, S.R. Whitton HKU(M)14078 (IFRD). NOTES: Rosellinia necatrix is characterised by ascomata that are tightly clustered together, short stipitate and surrounded by a well developed subiculum of Dematophora necatrix Hartig. The asci have a subapical apparatus 6.5–10 µm high, 5–6.5 µm upper width and 2.7–5.5 µm lower width. The ascospores are 30–47 × 5.5–8 µm, fusiform, brown, surrounded by a thin gelatinous sheath and have a germ slit 7–22 µm long (Petrini, 1992). Ascomatal morphology, especially the presence of a short stipe, the presence of copious anamorph surrounding the ascomatal base, and the asci and the ascomatal morphology and dimensions make placement of this specimen in R. necatrix appropriate. Ascospore size in this specimen is slightly longer than that described for R. necatrix by Francis (1985) and Petrini (1992); this is assumed to be due to natural variation.
MATERIAL EXAMINED: NEW ZEALAND, North Island, Auckland Region, Hunua Ranges, Wairoa-Cosseys Track, on decaying leaves of Freycinetia banksii, 9 May 1996, S.R. Whitton HKU(M)12854 (IFRD218-005). NOTES: Trichosphaerella decipiens is characterised by small (60–125 µm wide), globose, sessile perithecia, which have a periphysate ostiole. The ascomata have a few setae (18–90 µm long, 3.5–10 µm wide at the base), and the associated anamorph is Acremonium-like. The asci are 20–42 × 4–10 µm and the ascospores disarticulate at maturity into part-spores (2–3 µm diam) Samuels and Barr (1997) for T. decipiens; no taxonomically significant differences were observed.

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