Lasionectria sylvana (Mouton) Rossman & Samuels 1999
Details
Biostatus
Nomenclature
Classification
Associations
Descriptions
CONIDIAL STATE: Acremonium sp.
Mycelium white, sparse, spreading over the substrate or limited to a fringe of white to ferrugineous hyphae around the base of each perithecium. Perithecia solitary or in scattered groups of 3-4, white at first, becoming orange-brown; hirsute, hairs often forming an apical disc; urniform, 150-200 (-325) µm high x (187-) 220-280 (-374) µm wide, becoming collabent or not collapsing when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 20-30 (-40) µm thick. Surface view: cells prosenchymatous, walls 1-2 µm thick; pigmented. Longitudinal section: cells elliptical to flattened, 4-6 µm long, walls 1-2 µm thick, pigmented, becoming progressively more flattened and thin-walled toward the interior. Perithecial hairs composed of a solitary hypha or a triangular fasicle of tightly bound hyphae.70-200 µm long, 30-40 µm wide basally, orange to brown; hyphae unbranched, septate, 3-4 µm wide, walls c. 1 µm thick, pigmented. Hairs scattered over the upper half of the perithecia) wall or rising in one plane from the perithecia) apex to form a disc 400-500 µm in diam. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the perithecia) wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, (30-) 45-70 (-85) x (6-) 7-9 (-11) µm, 8-spored; apices flat, with an indistinct ring; bases pointed to rounded; ascospores uniseriate or biseriate, forming throughout the length. Ascospores fusiform-ellipsoidal, (9-) 11-15 (-17) x (2.5-) 3-4 (-5) µm, equally 2-celled, 2 drops in each cell, smooth to striate or punctate striate, non-constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, one short germ tube arising from each end of each ascospore. Colonies (2-) 4 cm diam., translucent to opaque, white; aerial mycelium sparse to cottony; hyphal, rope-like strands often forming; margin slightly undulate, non-aromatic, medium non-pigmented. ME: 1-1.5 (-2) cm diam., opaque; white, becoming pink to salmon; aerial mycelium lacking or loose and cottony or as hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. OA: 2.5-4 cm diam., opaque; white to pale pink at the margin, white to tan to ferrugineous in the centre; aerial mycelium loose and cottony or forming concentric rings of erect, outwardly directed, hyphal, rope-like strands; margin entire, undulate or rarely dissected, non-aromatic, medium non-pigmented. WAS: 3-5 cm diam., transparent; aerial mycelium sparse, undulate, white; margin entire to slightly undulate, non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS from the agar surface and from the aerial mycelium; monophialidic, unbranched or rarely verticillately branched, 0-2 (-3) septate, straight or undulate below the tip, smooth, (40-) 45-55 (-78) µm long, (2-) 2.5-3 (-4) µm wide basally, subtending hypha 1.5-2 (-2.5) µm wide. A solitary phialide constituting the terminal (17-) 37-50 (-54) µm of each phialophore, 1-2 µm wide at the unflared tip. Conidial production abundant. Phialoconidia ellipsoidal to nearly cylindrical, rarely elongating, (3-) 5-7 (-11) x (1.5-) 2-3 µm, unicellular, without a basal abscission scar, hyaline. Arising in basipetal succession; held in a solitary, hyaline drop of liquid.
Isolates of N. sylvana grow more rapidly on CMD than on ME, whereas the only isolate of N. vulpina known to me (Samuels 74-37) grows slowly and at the same rate on both media, this isolate was strongly aromatic whereas only one of 10 (Samuels 74-40) isolates of N. sylvana was aromatic.
When the perithecial hairs of N. sylvana are in the form of an apical disc, the perithecia resemble those of N. peristomialis and N. dentifera. The three species are easily distinguished by their ascospores, characteristics in culture, and conidial states.
Because the ascospores of N. sylvana usually contain four drops, they may appear to be multiseptate and one could easily identify the fungus as a Calonectria de Notaris, which is characterised by its phragmosporous ascospores. In studying the type specimen of C. fimbriata I saw only bicellular ascospores; the specimen is unquestionably N. sylvana.
The original description of Nectria peristomata, the only species included in Nectria subg. Zimmermannia Saccardo, suggests that this species is synonymous with N. sylvana.
Gamundi (1974) has recorded and illustrated N. sylvana (as Neohenningsia stellatula) from Argentina.