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Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999

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Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999
Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999

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Endemic
Present
New Zealand
Political Region

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Rossman & Samuels
Dingley
(Dingley) Rossman & Samuels
1999
30
ICN
NZ holotype
species
Hydropisphaera cyatheae

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Perithecia scattered or gregarious on a thin effused, hyaline subiculum not more than 20 µ thick; perithecia globose, pezizoid when dry, 0.2-0.3 mm diameter, salmon pink, translucent, smooth, sometimes hairy; perithecial wall pseudoparenchymatous 40 µ thick, cells 4-6 x 5-10 µ, thin walled, lightly pigmented, subhymenial layer pigmented but diffluent. Asci clavate or elliptical, thin walled 50-75 x 8-10 µ, 6-8 spored, arranged biseriately in ascus, pseudoparaphyses diffluent. Spores uniseptate, elliptical, or fusiform, ends truncated, 14-20 x 4-6 µ, walls smooth, hyaline.
Perithecia in subiculo tenui, effuso ad 20 µ crasso sparsa globosa, 0.2-0.3 mm diam. salmonea, translucentia, laevia sed interdum pilosa; pariete pseudoparenchymato 40 µ crasso, cellulis 4-6 x 5-10 µ, parietibus tenuibus leviter flavo tinctis, regione subhymenii tincta, diffluenti. Asci clavati vel elliptici, parietibus tenuibus 50-75 x 8-10 µ, 6-8 sporis, biseratis, pseudoparaphysibus diffluentibus. Sporae uniseptatae, ellipticae vel fusiformes, apice truncato, 14-20 x 4-6 µ, laeves, hyalinae.
When fresh, perithecia are translucent and hairy, with a papillate ostiole; in external appearance they resemble those of N. suffulta Berk. & Curt. but spores are larger and arranged biseriately. Morphologically this species agrees with the description of N. leucotrichia Penz. & Sace. which Weese (1914) treated as a synonym of N. suffulta. Unfortunately authentic material has not been available for study, consequently the New Zealand species is described as new.
Type collection: on stipes of Cyathea medullaris Swartz, Auckland, Waitakere Ra., Anawhata Rd., April 1948 (6201).

Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999

Type: Corticolous Fungi; Description: Ascomata stromatic, perithecial, gregarious, globose, salmon pink to orange, hairy, 0.2–0.3 mm in diameter, ostiole short papillate, superficial, on a thin, hyaline subiculum; on bark on stipes. Asci clavate, 50–75 × 8–10 μm. Ascospores elliptical to fusiform, 1-septate, 14–25 × 4–7 μm, smooth or finely striate, hyaline. Conidia (in culture only) oblong-elliptical, 0-septate, 6–9 × 2–3 μm, smooth, hyaline, held in a drop of liquid.
Distribution: Northland, Auckland, Wellington.; 1st Record: Dingley (1956: as Nectria cyatheae).
Significance: None. Probably saprobic.; Host(s): Astelia sp., Cyathea medullaris.
ADDITIONAL SPECIMENS EXAMINED: NEW ZEALAND: Auckland Province, Manukau County, Moumoukai Valley, Hunua Rd, on rachis of Cyathea medullaris, Dingley, Jun. 1949 (PDD 7461, 7462); Waitemata County, Henderson, Stony Creek, on rachis of Cyathea medullaris, Dingley, May 1948 (PDD 6202, 6203) ; Oratia, on rachis of Cyathea medullaris, Dingley, Jul. 1951 (PDD 10904); Titirangi, on rachis of Cyathea medullaris, Dingley, Jun. 1953 (PDD 12798), 9 Mar. 1966 (PDD 25134); Waitakere Ranges, off Anawhata Rd, on rachis of Cyathea medullaris, Dingley, Jun. 1954 (PDD 13846); off Anawhata Rd, Chateau Mosquito Track, on rachis of Cyathea medullaris, Dingley et al., 30 Oct. 1973 (PDD 32033, Samuels 73-234) ; Piha Valley, on rachis of Cyathea medullaris, Dingley, 20 Jun. 1970 (PDD 28646) ; vic. Waiatarua, on rachis of Cyathea medullaris, Dingley, 29 Jun. 1958 (PDD 18395); vic. Waiatarua, Rangemore Track, on rachis of Cyathea medullaris, Dingley, 8 May 1963 (PDD 21771) ; Rangemore Track, on rachis of Cyathed medullaris, Samuels 74-102, 10 Jul. 1974 (PDD 32562), Samuels 74-103 (PDD 32563), Samuels 74-106 (PDD 32561) ; Waitakere Ranges, West Coast Rd, Home Track, on rachis of Cyathea medullaris, Samuels 74-98, 10 Jul. 1974 (PDD 32564).

CONIDIAL STATE: Acremonium sp.

Mycelium ferrugineous hyphae surrounding the base of each perithecium, not apparent elsewhere. Perithecia gregarious, orange and forming extensive orange areas on the substrate, hirsute, urniform, 250-300 µm high x (310-) 375-440 (-530) µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 60-75 (-100) µm wide. Surface view: cells circular, (10-) 15-20 (-30) µm in greatest dimension, thin-walled. Longitudinal section: outer region 45-80 µm wide. cells circular, 20-30 µm in greatest dimension, thin-walled. Inner region 15 µm wide, cells flattened and compacted. Perithecial hairs triangular, (60-) 90-120 (-150) µm long x 60-70 µm basally, orange; arising from the surface of the perithecial wall and composed of a fascicle of unbranched, septate, smooth, thin-walled, 3 µm-wide hyphae. Perithecial papilla short, acute, formed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. A.sci clavate, (50-) 60-70 (-80) x (6-) 12-14 (-20) µm, 8-spored; apices rounded, simple; bases pointed; ascospores biseriate, forming throughout the length. Ascospores ellipsoidal to fusiform, 17-23 (-29) x 5-7 µm, equally 2-celled, striate or smooth, not constricted or slightly constricted at the septum, hyaline.

CHARACTERISTICS IN CULTURECMD: Ascospores germinating in low percentage overnight; a single germ tube arising from each end of each ascospore. Colonies 3-5 cm diam., opaque or rarely translucent, grey to white, aerial mycelium sparse to abundant and loose with faint concentric rings of erect, outwardly directed hyphae, margin undulate, non-aromatic, medium nonpigmented. ME: 1 cm or less diam., opaque, white, aerial mycelium lacking or sparse, margin undulate, nonaromatic, medium non-pigmented. OA: 7 cm diam., opaque, white to tan, aerial mycelium dense, with concentric rings of erect, outwardly directed hyphae and hyphal rope-like strands, margin undulate, non-aromatic, medium nonpigmented. WAS: 4-8 cm diam., translucent, nearly invisible, grey, aerial mycelium sparse or lacking, margin entire to slightly undulate, non-aromatic, medium non-pigmented. Perithecia forming within 6 weeks in cultures from single ascospores. Phialophores forming only on CMD, OA, and WAS after 2-4 weeks from aerial mycelium, unbranched, monophialidic, aseptate, straight to sinuous, smooth, 18-43 µm long, 2-3 µm wide basally, 1-1.5 cm wide at the unflared tip. Conidial production sparse. Phialoconidia oblong-elliptical, (5.5-) 6-9 (-1,3) x 2-2.5 (-3) µm, unicellular, hyaline, with or without a slightly protuberant, flattened, basal abscission scar. Arising in basipetal succession, held in a single, terminal, hyaline drop of liquid.

HABITAT: On rachis of Cyathea medullaris
NOTES: Superficially N. cyathea resembles N. sylvana and N. vulpina, both of which are also found on fern rachises. The perithecial wall of N. cyathea is much wider than that of either N. sylvana or N. vulpina; cells of the surface of the perithecial wall of N. cyathea are thin-walled and nearly angular, not thick-walled and prosenchymatous.
Nectria cyathea and N. suffulta are closely related. N. suffulta is heterothallic and its perithecia, asci, and ascospores are smaller than those of the homothallic N. cyathea. N. suffulta is a common tropical species (Samuels 1976) and is not known from New Zealand. A recent collection (PDD 32561) from New Zealand matched N. suffulta very well in morphological detail, but the isolate was homothallic and the ascospore measurements ranged from 13-22 x 4-7 µm, encompassing the measurements of both species. Ascospores produced in cultures of N. suffulta often have the measurements of the ascospores of N. cyathea, 10 µm longer than is usual for N. suffulta. Perithecia produced in culture from isolates from four collections of "typical" N. cyathea contained eight large ascospores.
HOLOTYPE: NEW ZEALAND: Auckland Province Waitemata County, Waitakere Ranges, Anawhata Rd, on rachis of Cyathea medullaris, Dingley, Apr. 1948 (PDD 6201).

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Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999
Hydropisphaera cyatheae (Dingley) Rossman & Samuels (1999)
Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999
Hydropisphaera cyatheae (Dingley) Rossman & Samuels (1999)
Hydropisphaera cyatheae (Dingley) Rossman & Samuels 1999
Hydropisphaera cyatheae (Dingley) Rossman & Samuels (1999)

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1cb1b342-36b9-11d5-9548-00d0592d548c
scientific name
Names_Fungi
1 January 2001
9 January 2003
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