Samuels, G.J. 1976: Perfect states of Acremonium. The genera Nectria, Actiniopsis, Ijuhya, Neohenningsia, Ophiodictyon, and Peristomialis. New Zealand Journal of Botany 14(3): 231-260.
Details
Descriptions
CONIDIAL STATE: Acremonium sp.
Mycelium a fringe of white hyphae surrounding the base of each perithecium, not visible elsewhere, Perithecia solitary; at first white, becoming orange, glabrous and scaly, globose to tympaniform 250-310 tom diam., becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 50-65 µm thick. Surface view: cells pseudoparenchymatous, 7-15 µm in greatest, dimension, walls 0.5-1.0 µm thick. Longtitudinal section: outer region 20-30 µm wide, cells elliptical, 5-15 µm long, 3-5 µm wide, walls 0.5-1.0 µm thick and pigmented; inner region 20-30 µm wide, cells elongated and compressed, outline of individual cells not apparent, walls 1-2 µm thick. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the Perithecial wall; ostiolar canal periphysate. Asci clavate, 60-80 (-100) x (9-) 10-18 An, 8-spored; apices rounded, immature asci appearing to have a pore that is not visible at maturity; bases rounded, ascospores biseriate, forming throughout the length. Ascospores fusiform, straight or curved, (13-) 19-22 (-24) x (3.5-) 4-1.5 (-5) µm, equally 2-celled, 4 striate, not constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating in low percentage overnight. Colonies 4-5 cm diam., transparent, white, aerial mycelium sparse, margin entire, aromatic, medium non-pigmented. Perithecia forming within 6-8 weeks in cultures derived from single ascospores. ME: 2.5-3 cm diam., barely transparent to translucent, white, aerial mycelium sparse, margin entire, aromatic, medium non-pigmented. OA: 3 cm diam., opaque; white, becoming pale pink; aerial mycelium dense, forming hyphal, rope-like strands; margin entire to slightly undulate, aromatic, medium non-pigmented. Perithecia forming within 6-8 weeks in cultures derived from solitary ascospores. WAS: 3 cm diam., transparent, aerial mycelium sparse, margin entire, non-aromatic, medium non-pigmented. Perithecia forming within 6 weeks in cultures derived from solitary ascospores. Phialophores arising on CMD, ME, OA, and WAS, forming from the surface of the agar and from the aerial mycelium; monophialidic, unbranched, 1-3 septate, straight, smooth, 70-110 jm long, 3-4 µm wide basally, subtending hypha 3-4 µm wide. A solitary phialide constituting the terminal 50-60 (-80) µm of each phialophore, 2-3 µm wide at the unflared or slightly flared tip. Conidial production sparse. Phialoconidia allantoid, elliptical to nearly cylindrical, (7-) 10-12 (-13) x (2.5-) 3-3.5 (-4) µm, unicellular, hyaline, with a slightly protuberant, flattened, basal abscission scar. Arising in basipetal succession, held in a solitary drop of hyaline liquid.
CONIDIAL STATE: Acremonium sp.
Mycelium ferrugineous hyphae surrounding the base of each perithecium, not apparent elsewhere. Perithecia gregarious, orange and forming extensive orange areas on the substrate, hirsute, urniform, 250-300 µm high x (310-) 375-440 (-530) µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 60-75 (-100) µm wide. Surface view: cells circular, (10-) 15-20 (-30) µm in greatest dimension, thin-walled. Longitudinal section: outer region 45-80 µm wide. cells circular, 20-30 µm in greatest dimension, thin-walled. Inner region 15 µm wide, cells flattened and compacted. Perithecial hairs triangular, (60-) 90-120 (-150) µm long x 60-70 µm basally, orange; arising from the surface of the perithecial wall and composed of a fascicle of unbranched, septate, smooth, thin-walled, 3 µm-wide hyphae. Perithecial papilla short, acute, formed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. A.sci clavate, (50-) 60-70 (-80) x (6-) 12-14 (-20) µm, 8-spored; apices rounded, simple; bases pointed; ascospores biseriate, forming throughout the length. Ascospores ellipsoidal to fusiform, 17-23 (-29) x 5-7 µm, equally 2-celled, striate or smooth, not constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURECMD: Ascospores germinating in low percentage overnight; a single germ tube arising from each end of each ascospore. Colonies 3-5 cm diam., opaque or rarely translucent, grey to white, aerial mycelium sparse to abundant and loose with faint concentric rings of erect, outwardly directed hyphae, margin undulate, non-aromatic, medium nonpigmented. ME: 1 cm or less diam., opaque, white, aerial mycelium lacking or sparse, margin undulate, nonaromatic, medium non-pigmented. OA: 7 cm diam., opaque, white to tan, aerial mycelium dense, with concentric rings of erect, outwardly directed hyphae and hyphal rope-like strands, margin undulate, non-aromatic, medium nonpigmented. WAS: 4-8 cm diam., translucent, nearly invisible, grey, aerial mycelium sparse or lacking, margin entire to slightly undulate, non-aromatic, medium non-pigmented. Perithecia forming within 6 weeks in cultures from single ascospores. Phialophores forming only on CMD, OA, and WAS after 2-4 weeks from aerial mycelium, unbranched, monophialidic, aseptate, straight to sinuous, smooth, 18-43 µm long, 2-3 µm wide basally, 1-1.5 cm wide at the unflared tip. Conidial production sparse. Phialoconidia oblong-elliptical, (5.5-) 6-9 (-1,3) x 2-2.5 (-3) µm, unicellular, hyaline, with or without a slightly protuberant, flattened, basal abscission scar. Arising in basipetal succession, held in a single, terminal, hyaline drop of liquid.
Nectria cyathea and N. suffulta are closely related. N. suffulta is heterothallic and its perithecia, asci, and ascospores are smaller than those of the homothallic N. cyathea. N. suffulta is a common tropical species (Samuels 1976) and is not known from New Zealand. A recent collection (PDD 32561) from New Zealand matched N. suffulta very well in morphological detail, but the isolate was homothallic and the ascospore measurements ranged from 13-22 x 4-7 µm, encompassing the measurements of both species. Ascospores produced in cultures of N. suffulta often have the measurements of the ascospores of N. cyathea, 10 µm longer than is usual for N. suffulta. Perithecia produced in culture from isolates from four collections of "typical" N. cyathea contained eight large ascospores.
CONIDIAL STATE: Acremonium sp.
Mycelium not visible. Perithecia solitary. yellow to orange, globose, 200-250 µm diam., with an apical, stellate crown of hairs, becoming collabent or not collapsing when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 20-25 µm thick. Surface view: cells prosenchymatous, walls 1-2 µm thick. Longitudinal section: outer region 10-15 µm wide, cells prosenchymatous, walls of cells 1-2 µm thick; inner region 5-10 µm wide, cells ellipsoidal to flattened, 5-15 µm long x 2-3 µm wide, walls c. 1 µm thick and pigmented, cells becoming progressively more flattened toward the interior. Perithecial hairs fasciculate, forming a yellow, stellate, apical disc, 150-200 µm diam.; composed of unbranched, septate, tightly bound hyphae, 3-4 µm wide with walls 1-2 µm thick; arising from the prosenchymatous region of the outer perithecial wall. Perithecial papilla lacking or short and acute, ostiolar opening formed by narrow, hyphal elements arising from the inner region of the Perithecial wall; ostiolar canal periphysate. Asci cylindrical, 45-55 x 5-6 µm, 8-spored; apices rounded, with a ring; bases rounded; ascospores uniseriate, forming throughout the length. Ascospores elliptical to fusiform-elliptical, 6-8 (-9) x 3-4 µm, equally 2-celled with one drop in each cell, finely spinulose., not constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, one short germ tube arising from each cell. Colonies 2-3.5 cm diam., translucent to opaque, white to pale pink, aerial mycelium loose and cottony, margin undulate, non-aromatic, medium non-pigmented. ME: 1-1.5 cm diam., opaque, white to pale pink; aerial mycelium as erect, outwardly directed, hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. OA: 2-2.5 cm diam., opaque, white; aerial mycelium as hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. WAS: 4 cm diam., transparent, white, aerial mycelium sparse, margin undulate, non-aromatic, medium non-pigmented. Phialophores forming on CMD, ME, OA, and WAS, arising from the surface of the agar and from the aerial mycelium; monophialidic, or irregularly and frequently branched, 0-2 septate, straight, smooth. 22-58 µm long, 2-3 µm wide basally, subtending hypha 2-3 µm wide. A solitary phialide constituting the terminal (9-) 20-40 (-50) µm of each phialophore, 1-1.5 µm wide at the unflared tip. Phialoconidia globose, 2-3 µm diam., to broadly elliptical, 3-4 x 2.5-3 µm, unicellular, hyaline, with a slightly protuberant, flattened, basal abscission scar. Arising in basipetal succession and held in a solitary drop of hyaline liquid.
CONIDIAL STATE: Acremonium sp.
Mycelium white, forming a ring around the base of each perithecium, not visible apart from the perithecia. Perithecia solitary or in caespitose clusters of up to 10, orange, globose, 260-370 µm diam., becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 50-80 µm wide. Surface view: cells obscured by the hyphal covering. Longitudinal section: outer region 40-60 µm wide, cells prosenchymatous to loose and hyphal, walls c. 3 µm thick, cells becoming progressively more compacted toward the interior of the perithecial wall. Inner region 15-20 µm wide; cells elliptic, 5-7 µm long x 2-3 µm wide, walls 1-2 µm thick and pigmented; cells becoming progressively more flattened, thin-walled, and non-pigmented toward the interior. Hyphal covering white, composed of branched septate, spinulose, straight, flexuous, 2-3-µm-wide hyphae with walls 0.5-1.0 µm thick, growing outwardly from the surface of the perithecial wall to a length of 40-60 µm, many free ends visible. Perithecial papilla lacking; ostiolar canal formed by narrow hyphal elements arising from the inner region of the perithecial wall, periphysate. Asci clavate, 100-110 x (8-) 11-17 µm, 8-spored; apices rounded, simple; bases rounded; ascospores biseriate, forming throughout the length. Ascospores fusiform, straight or slightly curved, 26-32 x 6-7 (-8) µm, equally 2-celled, two to three drops in each cell, striate, non-constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating in high percentage overnight, 3-4 germ tubes arising laterally from each ascospore. Colonies 2.5-3 cm diam., translucent, pale pink, aerial mycelium sparse, margin undulate, non-aromatic, medium non-pigmented. ME: 1 cm diam., opaque, white; aerial mycelium as loose, erect, outwardly directed hyphae; margin undulate, non-aromatic, medium non-pigmented. OA: 2.5 cm diam., opaque, pale pink, aerial mycelium compacted, margin slightly undulate, non-aromatic, medium non-pigmented. WAS: 2.5 cm diam., transparent, white, aerial mycelium sparse, loose, forming indistinct concentric rings, margin entire, non-aromatic, medium non-pigmented. Phialophores forming only on CMD from the surface of the medium and from the aerial mycelium, monophialidic unbranched or branched once, 4-6 septate, straight, coarsely spinulose, 60-90 µm long, 3 µm wide basally, subtending hypha 3 µm wide. A solitary, coarsely spinulose phialide constituting the terminal (23-) 35-40 (-50) µm of each phialophore, 2 µm wide at the unflared tip. Conidial production sparse after 2 weeks, but abundant after 6 weeks. Phialoconidia ellipsoidal, 6.5-9.5 (-11) x (2-) 3-3.5 (-4) µm, unicellular, hyaline, with or without a protuberant, flattened, basal abscission scar. Arising in basipetal succession and held in a single, terminal, hyaline drop of liquid.
Nectria freycinetii and N. foliicola are closely related. The perithecial wall of N. foliicola is orange to brown and the perithecial hairs are tan and smooth; the asci measure (51-) 60-80 (-85) x 10-12 (-16) µm and each has a simple apex; the fusiform ascospores measure (17-) 21-27 (-30) x (4-) 5 µm and are striate. A conidial state has not been recorded for N. foliicola and conidia did not form in cultures made from South american material (Samuels 1976). Perithecia formed in cultures derived from solitary ascospores on OA and CMD. I did not see conidia on any of six, including Holotype (K) and Isotype (FH), fresh or dried neotropical collections of this species. N. foliicola is apparently known only from the neotropics and has been described and illustrated by Samuels (1976).
CONIDIAL STATE: Acremonium sp.
Mycelium flat, white, spreading slightly away from the perithecia or restricted as a white fringe surrounding the base of each perithecium. Perithecia solitary, in groups of a few or densely gregarious and forming extensive orange areas; at first white, becoming yellow, then orange; wall smooth to granular, dull; urniform, 140-180 µm high x (150-) 200-220 (-280) µm wide, becoming collabent when dry, ferrugineous in 3% potassium hydroxide. Perithecial wall 15-20 (-25) µm thick. Surface view: cells angular to prosenchymatous, 5-10 µm in greatest dimension, walls 1-2 µm thick and pig mented. Longitudinal section: cells elliptic to flattened, 5-7 µm long, 2-3 µm wide, walls 1-2 µm thick and pigmented, cells becoming progressively more flattened toward the interior. Perithecial hairs composed of solitary or catenulate, short, globose to ellipsoidal cells with walls c. 1 µm thick; (10-) 20-30 (-50) µm long, arising from the surface of the perithecia) wall. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the perithecia) wall; ostiolar canal periphysate. Asci clavate, (35-) 45-56 x (6-) 7-9 µm, 8-spored; apices flat, with a frequently indistinct ring; bases rounded; ascospores biseriate, forming throughout the length. Ascospores fusiform to fusiform-ellipsoidal, (8-) 9-11 (-14) x 2.5-3 (-4) µm, equally 2-celled, 2 drops in each cell, smooth to punctate-striate to striate, not constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating in low percentage overnight or after 4 days; a single, short germ tube arising from each end of each ascospore. Colonies 1.5-2 cm diam., translucent, white; aerial mycelium sparse or as hyphal, rope-like strands; margin slightly undulate to dissected, aromatic, medium non-pigmented. ME: 1-1.5 cm diam., opaque, white; aerial mycelium as hyphal, rope-like strands; margin undulate, aromatic, medium non-pigmented. OA: 2-2.5 cm diam., opaque, white to light tan; aerial mycelium dense, flat; hyphal, rope-like strands frequently forming; aromatic; medium non-pigmented. WAS: 2 cm diam., transparent, grey to white. aerial mycelium sparse, margin entire., non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS, forming from the surface of the agar and from the aerial mycelium; monophialidic unbranched. 0 (-3) septate, straight, smooth, (31-) 35-45 (-50) µm long, 2-3 µm basally, subtending hypha 1.5-2 µm wide, A solitary phialide constitute ing the terminal (31-) 35-45 (-50) µm of each phialophore, 1-1.5 µm wide at the unflared tip. Phialoconidia ellipsoidal to nearly cylindrical 5-7 (-11) x 1.5-2.5 (-3) µm, unicellular, hyaline without a basal abscission scar. Arising in basipeta succession, held in a solitary, hyaline drop of liquid
CONIDIAL STATE: Acremonium sp.
Mycelium white or not visible. sparse, and growing away from the perithecia. Perithecia solitary or in groups of 2-4, hyaline with orange contents, globose, 200-370 µm diam., with an apical, stellate crown of hairs, not collapsing when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 30-40 µm thick. Surface view; cells prosenchymatous, walls 1-2 µm thick. Longitudinal section: outer region 10-20 µm wide, cells prosenchymatous, walls of cells 1-2 µm thick; inner region 15-20 µm wide, cells ellipsoidal, 3-7 µm long, walls 1-2 µm thick; cells becoming progressively more flattened toward the interior, merging with the cells of the prosenchymatous layer at the exterior. Perithecial hairs fasciculate, forming a stel.late apical disc or less frequently triangular and scattered, white, up to 200 µm long, 60-80 µm basally; composed of unbranched, septate, tightly bound, hyphae, c. 3 µm wide with walls c. 1 µm thick; arising from the prosenchymatous region of the outer perithecial wall. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periph ysate. Asci clavate, (52-) 70-85 (-105) x (8-) 9-13 (-15) µm, 8-spored; apices rounded, simple; bases rounded; ascospores biseriate, forming throughout the length. Ascospores fusiform, (18-) 25-32 (-37) x (3-) 4-6 (-7) µm. 1-2 (-5) septate; coarse striations ridge-like and frequently forming a blunt apiculus at the ends of the ascospores; not constricted or cells separating at the median septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight; a single, long, unbranched germ tube arising from each end of each ascospore. Colonies 1 cm diam., opaque, white to pale pink; aerial mycelium lacking or as hyphal, rope-like strands; non-aromatic, medium non-pigmented. ME: Less than 1 cm diam., opaque, white to pink; aerial mycelium sparse to lacking or as hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. OA: 1-2 cm diam., opaque, white to pale pink; aerial mycelium as hyphal, rope-like strands arising in concentric rings around the inoculum; non-aromatic, medium non-pigmented. WAS: 2 cm diam., transparent, grey, aerial mycelium sparse, margin undulate, non-aromatic, medium non-pigmented. Phialophores forming only on WAS, arising from the surface of the straw and from the aerial mycelium; monophialidic, unbranched, 0-2 septate, straight, smooth, (19-) 22-47 (-60) µm long, 2-3.5 µm basally, subtending hypha 2-3.5 µm wide. A solitary phialide constituting the terminal (7-) 23-33 µm of each phialophore, 1-1.5 µm wide at the unflared tip. Conidial production sparse. Phialoconidia ellipsoidal to nearly cylindrical. (4-) 5-7 (-8) x 2-2.5 µm, unicellular, hyaline, without a basal abscission scar. Arising in basipetal succession and held in a solitary, hyaline drop of liquid.
The apical fringe of hairs gives the perithecia `an apothecial aspect, thus explaining why the species has been treated as a discomycete.
CONIDIAL STATE: Acremonium sp.
Mycelium not apparent after perithecial formation. Perithecia solitary or in groups of from 3 to 25, each aggregate enclosed in white hyphae; orange, globose, 230-250 (-460) µm diam., becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 15-20 µm wide. Surface view: cells obscured by the hvphal covering. Longitudinal section: cells elliptical, 5-10 µm long x 4 wm wide.walls c. 1 µm thick, cells becoming progressively more flattened toward the interior of the perithecia) wall. Hyphal covering white, composed of long, branched, septate, smooth, wavy, flexuous, 5 µm wide hyphae with walls 1-2 µm thick; many free ends visible; hyphae around the ostiolar opening short. Hyphae of the covering arising from the surface of the perithecia) wall; covering completely enclosing 2 or more adjacent perithecia. Perithecial papilla short, acute, formed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, (45-) 70-80 (-103) x (5-) 9-12 (-19) µm, 8-spored; apices flat, simple; bases pointed or rounded; ascospores at first biseriate, becoming irregularly arranged in the upper two-thirds of each ascus. Ascospores ellipsoidal to elliptic-fusiform, (10-) 13-17 (-19) x (3-) 4-5 µm, equally 2-celled, with or without 2 drops in each cell, 3 or fewer complete striations visible in one plane of view, not constricted or slightly constricted at the septum, hyaline
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating in high percent age after 4 days. Colonies 2.5 cm diam., translucenn to opaque, white, aerial mycelium forming hypha rope-like strands, margin undulate to dissected, non aromatic, medium non-pigmented. Perithecia forming within 6 weeks in cultures from single ascospores ME: 1 cm or less diam., opaque, white to pal, pink or pale salmon, aerial mycelium as hypha rope-like strands, margin dissected. non-aromatic medium non-pigmented. OA: 3 cm diam., opaque, white, aerial myceliun as hyphal rope-like strands, surface of the colour wrinkled, margin entire to dissected, non-aromatic medium non-pigmented. WAS: 4-8 cm diam., translucent, grey; aerial mycelium sparse, white; margin undulate, non-aromatic, medium non-pigmented. Perithecia form ing within 6 weeks in cultures from single ascospores. Phialophores forming on CMD and WAS in 3-6 weeks, unbranched, monophialidic, 0-1 septate, straight, smooth, (16-) 20-30 (-35) µm long, 2-3 µm wide basally, subtending hypha 2-3 µm wide. A solitary phialide constituting the terminal 11-20 (-35) µm of each phialophore, 1-1.5 µm wide at the unflared tip. Conidial production sparse. Phialoconidia elliptical to nearly cylindrical, 4-7 (-10) x 1.5-2 µm, unicellular, hyaline, with or without a protuberant, flattened, basal abscission scar. Arising in basipetal succession, held in a single, terminal, hyaline drop of liquid.
CONIDIAL STATE: Acremonium sp.
Mycelium white, flat, spreading over the substrate. Perithecia densely gregarious in groups of up to 100, each aggregate enclosed in white hyphae; orange, globose, 220-375 µm diam., to broadly obpyriform, 200-300 µm high x 220-280 µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 30-50 µm thick. Surface view cells obscured by the hyphal covering. Longitudinal section: outer region 10-30 µm wide, cells prosenchymatous to hyphal, walls c. 1 µm thick, cells becoming progressively more compacted toward the interior of the perithecial wall. Inner region 15-20 µm wide, cells elliptical to flattened, 5-15 µm long, x 2-3 µm wide, walls c. 1 µm thick, cells becoming progressively more flattened and compacted toward the interior. Hyphal covering white, composed of branched, septate, smooth, straight to slightly wavy, flexuous, 2-3-um-wide hyphae with walls 1-2 µm thick; many free ends visible; hyphae around the ostiolar opening short. Hyphae of the covering arising from the surface of the perithecial wall; covering enclosing 2 or more adjacent perithecia. Perithecial papilla short, acute, composed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, (41-) 55-70 (-80) x (5.5-) 7-8 (-9) µm, 8-spored; apices rounded, with an indistinct ring; bases rounded to pointed; ascospores biseriate, forming throughout the length. Ascospores fusiform, allantoid or ellipsoidal, 9-12 (-14) x 3-5 µm, equally 2-celled, one drop in each cell, striate or punctate-striate, non-constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, a short germ tube arising from each end of each ascospore. Colonies 1.7-3 cm diam., translucent to opaque, white to pale pink, aerial mycelium loose, margin entire to undulate, non-aromatic; medium non-pigmented or rarely a pink pigment spreading through the medium. ME: 1 cm or less diam., opaque white to pale pink or salmon, aerial hyphae lacking to sparse and loose, margin undulate, non-aromatic, medium non-pigmented. OA: 2 cm diam., opaque, white at the margin, tan elsewhere, aerial mycelium consisting of a few white, hyphal, rope-like strands arising from the original inoculum, margin undulate, non-aromatic, medium non-pigmented. WAS: 1.5 cm diam., translucent, white, aerial mycelium scanty, margin undulate, non-aromatic, medium non-pigmented. Phialophores forming on CMD, ME, and WAS from the surface of the agar and from the aerial mycelium, unbranched, monophialidic, 0-1 septate, straight or sinuate at the tip, smooth, 30-70 µm long, 2-3 µm wide basally, subtending hypha 2-3 µm wide. A solitary phialide constituting the terminal (30-) 40-60 µm of each phialophore, 1-1.5 µm wide at the unflared tip. Conidial production abundant. Phialoconidia ellipsoidal to nearly cylindrical. 6-7 (-9) x 2-2.5 µm, many elongating to 10-19 µm, unicellular, hyaline, lacking a basal abscission scar. Arising in basipetal succession, held in a single, terminal, hyaline drop of liquid.
CONIDIAL STATE: Acremonium sp.
Mycelium not visible, or white and growing around the perithecial bases; hyphae unbranched, septate, thin-walled, c. 4 µm wide, arising from the cells of the base of the perithecia. Perithecia solitary to gregarious, yellow to orange, globose to urniform, 370-420 µm high x (250-) 370-430 (-550) µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 30-50 (-70) µm thick. Surface view: cells circular, 10-15 µm in greatest dimension, thin-walled. Longitudinal section: outer region 15-30 (-50) µm wide, cells circular, 10-15 µm in greatest dimension, thin-walled. Inner region 15 µm wide, cells flattened and compacted, thin-walled. Perithecial hairs lacking, or short, triangular, orange; composed of a fascicle of septate, unbranched, thin-walled hyphae. Perithecial papilla lacking or short and acute; formed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. Asci clavate, (40-) 60-75 (-100) x (5-) 8-10 (-14) µm, 8-spored; apices flat, simple; bases pointed; ascospores biseriate above, uniseriate below, forming throughout the length. Ascospores ellipsoidal, (9-) 11-14 (-17) x (3-) 5-6 µm, equally 2-celled, striate or smooth, not constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURECMD : 1-5 cm diam., opaque, white to pale pink or pale brown, sparse aerial mycelium, margin slightly undulate, non-aromatic, medium non pigmented, perithecia forming within 1 month, homothallic. ME: 0.5-2 cm diam., opaque, white to pale pink, aerial mycelium sparse, margin undulate, nonaromatic, medium non-pigmented. OA: 1.5-7 cm diam., opaque, white to tan, flat, with hyphal rope-like strands, margin slightly undulate, non-aromatic, medium non-pigmented, perithecia forming within 1 month, homothallic. WAS: 1 cm in diam., translucent, nearly invisible, aerial mycelium lacking, margin slightly undulate, non-aromatic, medium non-pigmented. Phialophores forming only on CMD and OA from surface of agar and aerial mycelium, unbranched, monophialidic, aseptate or rarely sep tate, straight, smooth, (23-) 30-40 (-65) µm long, 2-3 µm wide basally, 1-2 µm wide at the unflared tip. Phialoconidia ellipsoidal becoming elongated, (3.5-) 7-9 (-13) x 3-4 µm, unicellular, hyaline, without a basal abscission scar; arising in basipetal succession, held in a hyaline drop of liquid at the tip of each phialide.
Nectria peziza is common in the northern hemisphere and has many synonyms (Samuels 1976). It has been µmply described and illustrated by Booth (1959), Hanlin (1963), Gams (1971), and Samuels (1976).
Nectria peziza is closely related to N. suffulta. The usually smooth perithecial wall of the ascocarp of N. peziza is not as wide as the hirsute wall of N. suffulta. Short, inconspicuous hairs may occasionally arise from the perithecial wall of N. peziza, but they are apparent only under high magnification. N. peziza is temperate in distribution and homothallic whereas N. suffulta is tropical in distribution and heterothallic (Samuels 1976).
Superficially N. peziza resembles N. vulpina and N. manuka. It is readily distinguished from these two species by the thin-walled, globose cells that make up its much thicker perithecial wall.
According to Nannfeldt (1932) the type species of Perrotiella Naoumoff (P. uralensis Naoumoff), described as a discomycete, is N. peziza.
CONIDIAL STATE: Acremonium sp.
Mycelium white. flat, spreading over the substrate. Perithecia densely gregarious, aggregates enclosed in white hyphae, yellow, globose, 150-220 µm diam., becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 15-25 µm thick. Surface view: cells at the surface obscured by the hyphal covering. Longitudinal section: outer region 10-15 µm wide, cells prosenchymatous to loose and hyphal, walls c. l µm thick, cells progressively more compacted toward the interior of the perithecial wall. Inner region 5-10 µm wide, cells ellipsoidal to elongated, 5-7 µm long x 2-3 µm wide, walls c. 1 µm thick, cells becoming progressively more flattened and. thin-walled toward the interior. Hyphal covering white, composed of branched, septate, slightly wavy, flexuous, 2-3 µm wide, hyphae with walls 0.5-1.0 µm thick; growing out wardly from the surface of the perithecial wall, completely enclosing two or more adjacent perithecia, few free ends visible. Perithecial papilla lacking, ostiolar opening composed of narrow hyphal elements arising from the inner region of the perithecial wall, periphysate. Asci clavate, 60-70 x 9-10 µm, 8-spored; apices rounded, with an inconspicuous ring; bases pedicellate; ascospores biseriate, forming throughout the length. Ascospores ellipsoidal to fusiform-ellipsoidal, 10-14 x 3-4 µm, equally 2-celled, 2 drops in each cell; a tuberculate, non-staining sheath visible around each while still in the asci, ascospores tuberculate when discharged, but lacking a sheath; not constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight in low percentage. Colonies 3 cm diam., translucent, pale pink to white, aerial mycelium sparse, margin undulate, non-aromatic, medium non-pigmented. ME: 1 cm or less diam., opaque, white to pale pink or pale salmon; aerial mycelium as scattered, hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. OA: 2.5-3 cm diam., opaque, white to pale pink; aerial mycelium dense with erect, outwardly directed, hyphal, rope-like strands arising from the center; non-aromatic, medium non-pigmented. WAS: 2 cm diam., translucent, grey, aerial mycelium white, sparse, margin undulate, non-aromatic, medium non-pigmented. Phialophores forming on OA and WAS from hyphal, rope-like strands, the surface of the medium, and from straw; monophialidic, unbranched, 0-1 septate, straight, smooth or spinulose, 33-45 µm long, 2-3 µm basally, subtending hypha 2-3 µm wide. A solitary, smooth or spinulose phialide constituting the terminal (10-) 29-33 µm of each phialophore, 1.5-2 µm wide at the unflared tip. Phialoconidia ellipsoidal, (4-) 5-7 x 2-3 (-3.5) µm, unicellular, hyaline, with or without a protuberant, flattened, basal abscission scar. Arising in basipetal succession, held in a solitary drop of hyaline liquid.
CONIDIAL STATE: Acremonium sp.
Mycelium white, sparse, spreading over the substrate or limited to a fringe of white to ferrugineous hyphae around the base of each perithecium. Perithecia solitary or in scattered groups of 3-4, white at first, becoming orange-brown; hirsute, hairs often forming an apical disc; urniform, 150-200 (-325) µm high x (187-) 220-280 (-374) µm wide, becoming collabent or not collapsing when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 20-30 (-40) µm thick. Surface view: cells prosenchymatous, walls 1-2 µm thick; pigmented. Longitudinal section: cells elliptical to flattened, 4-6 µm long, walls 1-2 µm thick, pigmented, becoming progressively more flattened and thin-walled toward the interior. Perithecial hairs composed of a solitary hypha or a triangular fasicle of tightly bound hyphae.70-200 µm long, 30-40 µm wide basally, orange to brown; hyphae unbranched, septate, 3-4 µm wide, walls c. 1 µm thick, pigmented. Hairs scattered over the upper half of the perithecia) wall or rising in one plane from the perithecia) apex to form a disc 400-500 µm in diam. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the perithecia) wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, (30-) 45-70 (-85) x (6-) 7-9 (-11) µm, 8-spored; apices flat, with an indistinct ring; bases pointed to rounded; ascospores uniseriate or biseriate, forming throughout the length. Ascospores fusiform-ellipsoidal, (9-) 11-15 (-17) x (2.5-) 3-4 (-5) µm, equally 2-celled, 2 drops in each cell, smooth to striate or punctate striate, non-constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, one short germ tube arising from each end of each ascospore. Colonies (2-) 4 cm diam., translucent to opaque, white; aerial mycelium sparse to cottony; hyphal, rope-like strands often forming; margin slightly undulate, non-aromatic, medium non-pigmented. ME: 1-1.5 (-2) cm diam., opaque; white, becoming pink to salmon; aerial mycelium lacking or loose and cottony or as hyphal, rope-like strands; margin undulate, non-aromatic, medium non-pigmented. OA: 2.5-4 cm diam., opaque; white to pale pink at the margin, white to tan to ferrugineous in the centre; aerial mycelium loose and cottony or forming concentric rings of erect, outwardly directed, hyphal, rope-like strands; margin entire, undulate or rarely dissected, non-aromatic, medium non-pigmented. WAS: 3-5 cm diam., transparent; aerial mycelium sparse, undulate, white; margin entire to slightly undulate, non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS from the agar surface and from the aerial mycelium; monophialidic, unbranched or rarely verticillately branched, 0-2 (-3) septate, straight or undulate below the tip, smooth, (40-) 45-55 (-78) µm long, (2-) 2.5-3 (-4) µm wide basally, subtending hypha 1.5-2 (-2.5) µm wide. A solitary phialide constituting the terminal (17-) 37-50 (-54) µm of each phialophore, 1-2 µm wide at the unflared tip. Conidial production abundant. Phialoconidia ellipsoidal to nearly cylindrical, rarely elongating, (3-) 5-7 (-11) x (1.5-) 2-3 µm, unicellular, without a basal abscission scar, hyaline. Arising in basipetal succession; held in a solitary, hyaline drop of liquid.
Isolates of N. sylvana grow more rapidly on CMD than on ME, whereas the only isolate of N. vulpina known to me (Samuels 74-37) grows slowly and at the same rate on both media, this isolate was strongly aromatic whereas only one of 10 (Samuels 74-40) isolates of N. sylvana was aromatic.
When the perithecial hairs of N. sylvana are in the form of an apical disc, the perithecia resemble those of N. peristomialis and N. dentifera. The three species are easily distinguished by their ascospores, characteristics in culture, and conidial states.
Because the ascospores of N. sylvana usually contain four drops, they may appear to be multiseptate and one could easily identify the fungus as a Calonectria de Notaris, which is characterised by its phragmosporous ascospores. In studying the type specimen of C. fimbriata I saw only bicellular ascospores; the specimen is unquestionably N. sylvana.
The original description of Nectria peristomata, the only species included in Nectria subg. Zimmermannia Saccardo, suggests that this species is synonymous with N. sylvana.
Gamundi (1974) has recorded and illustrated N. sylvana (as Neohenningsia stellatula) from Argentina.
Mycelium white, scanty, spreading over the substrate. Perithecia solitary or in groups of a few, orange, glabrous or hirsute, urniform, (155-) 190-220 (-230) µm high x (200-) 247-270 (-375) µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 15-25 (-40) µm thick. Surface view: cells prosenchymatous, walls 1-2 µm thick. Longitudinal section: cells elliptical, 5-7 µm long x 2-4 µm wide, walls 1-2 µm thick, cells becoming progressively more flattened and thin-walled toward the interior. Perithecial hairs lacking or composed of from one to several fasciculate hyphae, 10-70 wm long, 20-40 µm wide basally, orange to brown; hyphae unbranched, septate, 3-5 µm wide with walls c. 1 µm thick, arising from the surface of the perithecial wall. Perithecial papilla lacking or very short, composed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, 37-58 x 6-8 (-10) µm, 8-spored; apices flat, with a ring; bases rounded; ascospores obliquely uniseriate, becoming irregularly arranged, forming throughout the length. Ascospores fusiform-ellipsoidal, (7-) 8-11 (-13) x 3-4 µm, equally 2-celled, 2 drops in each cell, striate, non-constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, one, short germ tube arising from one end of each ascospore. Colonies 1.5-2.0 cm diam., translucent to opaque, white to pale pink, aerial mycelium loose, abundant pink to salmon slime on the surface of the colony, margin entire to slightly undulate, aromatic, medium non-pigmented. ME: 1 cm or less diam., opaque, pink to salmon, aerial mycelium loose or forming poorly developed, hyphal, rope-like strands, abundant pink to salmon slime on the surface of the colony, margin undulate, aromatic, medium non-pigmented. OA: 1.5-2.5 cm diam., opaque; yellow to salmon in the centre, white elsewhere; aerial mycelium as erect, outwardly directed, hyphal, rope-like strands, margin undulate, aromatic, medium non-pigmented. WAS: 2 cm diam., translucent, aerial mycelium sparse, white, margin entire, non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS from the surface of the agar and from the aerial mycelium; monophialidic, unbranched, aseptate, straight, smooth, 49-57 µm long, 2.5-3 µm wide basally, subtending hypha 1.5-2 µm wide. A solitary phialide constituting the terminal 44-52 µm of each phialophore, 1-1.5 µm wide at the unflared tip. Conidial production abundant. Phialoconidia ellipsoidal to cylindrical, 5-7 x 1.5-2 µm, unicellular, hyaline, lacking a basal abscission scar. Arising in basipetal succession, held in a solitary pink to salmon (on CMD, ME, and OA) or hyaline (on WAS) drop of liquid.
Nectria vulpina has not previously been grown in pure culture. The above description of the characteristics in culture is based on colonies derived from three ascospores of one collection.
Cited scientific names
- Nectria arenuloides Samuels 1976
- Nectria cyatheae Dingley 1956
- Nectria dentifera Samuels 1976
- Nectria freycinetiae Samuels 1976
- Nectria manuka Dingley 1951
- Nectria peristomialis (Berk. & Broome) Samuels 1976
- Nectria pertusa Pat. 1895
- Nectria pertusoides Samuels 1976
- Nectria peziza (Tode) Fr. 1849
- Nectria phormiicola Samuels 1976
- Nectria sylvana Mouton 1900
- Nectria vulpina (Cooke) Ellis 1882