Nectria manuka Dingley 1951
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Nectria manuka Dingley 1951
Nectria manuka Dingley 1951
Nectria manuka Dingley 1951
CONIDIAL STATE: Acremonium sp.
Mycelium flat, white, spreading slightly away from the perithecia or restricted as a white fringe surrounding the base of each perithecium. Perithecia solitary, in groups of a few or densely gregarious and forming extensive orange areas; at first white, becoming yellow, then orange; wall smooth to granular, dull; urniform, 140-180 µm high x (150-) 200-220 (-280) µm wide, becoming collabent when dry, ferrugineous in 3% potassium hydroxide. Perithecial wall 15-20 (-25) µm thick. Surface view: cells angular to prosenchymatous, 5-10 µm in greatest dimension, walls 1-2 µm thick and pig mented. Longitudinal section: cells elliptic to flattened, 5-7 µm long, 2-3 µm wide, walls 1-2 µm thick and pigmented, cells becoming progressively more flattened toward the interior. Perithecial hairs composed of solitary or catenulate, short, globose to ellipsoidal cells with walls c. 1 µm thick; (10-) 20-30 (-50) µm long, arising from the surface of the perithecia) wall. Perithecial papilla lacking, ostiolar opening formed by narrow hyphal elements arising from the inner region of the perithecia) wall; ostiolar canal periphysate. Asci clavate, (35-) 45-56 x (6-) 7-9 µm, 8-spored; apices flat, with a frequently indistinct ring; bases rounded; ascospores biseriate, forming throughout the length. Ascospores fusiform to fusiform-ellipsoidal, (8-) 9-11 (-14) x 2.5-3 (-4) µm, equally 2-celled, 2 drops in each cell, smooth to punctate-striate to striate, not constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating in low percentage overnight or after 4 days; a single, short germ tube arising from each end of each ascospore. Colonies 1.5-2 cm diam., translucent, white; aerial mycelium sparse or as hyphal, rope-like strands; margin slightly undulate to dissected, aromatic, medium non-pigmented. ME: 1-1.5 cm diam., opaque, white; aerial mycelium as hyphal, rope-like strands; margin undulate, aromatic, medium non-pigmented. OA: 2-2.5 cm diam., opaque, white to light tan; aerial mycelium dense, flat; hyphal, rope-like strands frequently forming; aromatic; medium non-pigmented. WAS: 2 cm diam., transparent, grey to white. aerial mycelium sparse, margin entire., non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS, forming from the surface of the agar and from the aerial mycelium; monophialidic unbranched. 0 (-3) septate, straight, smooth, (31-) 35-45 (-50) µm long, 2-3 µm basally, subtending hypha 1.5-2 µm wide, A solitary phialide constitute ing the terminal (31-) 35-45 (-50) µm of each phialophore, 1-1.5 µm wide at the unflared tip. Phialoconidia ellipsoidal to nearly cylindrical 5-7 (-11) x 1.5-2.5 (-3) µm, unicellular, hyaline without a basal abscission scar. Arising in basipeta succession, held in a solitary, hyaline drop of liquid