Growth in YM broth: After 5 days at 17° C, the vegetative cells are oval or short oval, 3.0-4.5 x 4.0-7.0 fim, single or in pairs (Fig. 1A). After one month at 17° C, a sediment and a thin pellicle are present.
Growth on YM agar: After one month at 17° C, the streak culture is grayish yellow, smooth, shining, mucous, soft and has an entire margin. Dalmau plate culture on corn meal agar: Pseudomycelium and true mycelium are formed. Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are round, 2.0-2.5 x 2.0-3.0 µm (Fig. IB). Fermentation: Negative.

Maximum growth temperature: 27-28° C. Vitamins required: Nil.
Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Positive.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blueB reaction: Positive.
G + C content of nuclear DNA: 49.8 mol% (by HPLC), 46.9 mol% (from 7rn) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-51, which was isolated from a dead leaf and a fruit of Coprosma tenuifolia, at Kaimanawa State Forest Park, Taupo, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8936.
B. coprosmaensis shows a close resemblance to B. pseudoalba in its biochemical and physicological characters, and also has similar G + C contents to B. globospora. However, the differences were found between B. coprosmaensis and the latter two species DNA dissimilarity as deduced from DNA-binding experiments (3-12%) (Table 5). It clearly indicates that B. coprosmaensis is a different species from B. globospora and B. pseudoalba.
B. coprosmaensis can be discriminated from B. pseudoalba in the assimilation of nitrite and from B. globospora in the assimilation of melibiose and ethanol (Table 6).

In liquid YM post dies 5 ad 17° C, cellulae vegetativae ovoideae vel subovoideae, 3.0-4.5 x 4.0-7.0 pm, singulae aut binae. Post unum mensem ad 17° C, sedimentum et pellicula formantur. Cultura in agaro YM, griseo-fiava, glabra, niti-da, mucosa, mollis et margine glabra. Ballistosporae rotundae, 2.0-2.5 x 2.0-3.0 pm. Pseudomycelium et mycelium formantur. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalosum, melibiosum, raffinosum, melezito¬sum, amylum solubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum, L-rhamnosum, ethanolum, glycerolum (exiguum, vel lente et exiguum), ery-thritolum, ribitolum (lente et exiguum), galactitolum, D-mannitolum, a-methyl-D-glucosidum, salicinum, glucono-5-lactonum (exiguum, vel lente et exiguum), acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum DL-Iacticum, acidum succinicum, acidum cit-ricum (exiguum) et inositolum assimilantur, at non L-sorbosum, lactosum, inulinum nec D-glucitolum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 27-28° C. Ad crescentiam vitaminum non necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucleico: 49.8 mol% (per HPLC), 46.9 mol% (ex 7m). Ubiquinonummajus: Q-10. Xylo-sum in cellulis presens.
Holotypus: JCM 8936 (originaJiterutNZ-51).

Growth in YM broth: After 5 days at 17° C, the vegetative cells are short oval to oval, sometimes ellipsoidal, 2.5^.0 x 4.0-6.0 µm (Fig. 2A). After one month at 17° C, a ring, islets and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is yellowish white to light yellow, smooth, shining, soft, and has an entire margin. Dalmau plate culture on corn meal agar: Mycelium and pseudomycelium are not formed. Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are round, napiform, turbinate or ampulliform, 2.2-3.2 x 2.0-4.0 ^m (Fig. 2B). Fermentation: Negative.

Maximum growth temperature: 22-23° C. Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative. Production of starch-like substances: Negative.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blueB reaction: Positive.
G + C content of nuclear DNA: 44.5 mol% (by HPLC), 42.7 mol% (from 7m) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-69, which was isolated from a dead leaf of Pseudowintera colonata infected with sooty moulds at Kaiwanawa State Forest, Paupo, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8937.
B. hannae has similar assimilation pattern to B. mrakii and has mol% G + C of 44.5 which is the similar to B. huiaensis. However, differences were found between B. hannae and the latter two species in the DNA dissimilarity as deduced from DNA-binding experiments (5-10%) (Table 7). It clearly indicates that B. hannae is a different species from B. huiaensis and B. mrakii.
Practically, B. hannae can be discriminated from B. huiaensis in the lack of assimilation of cadaverine and from B. mrakii in the lack of assimilation of salicin (Table 8)

In liquid YM post dies 5 ad -17° C, cellulae vegetativae subovoideae, ovoideae vel ellipsoideae, 2.5-4.0 x 4.0-6.0 µm. Post unum mensem ad 17° C, anulus, insulae et sedimentum formantur. Cultura in agaro YM, flavo-albida aut subflava, glabra, nitida, mollis et margine glabra. Ballistosporae rotundae, ampulliformes aut turbinatae, 2.2-3.2 x1 2.0-4.0 µm. Pseudomycelium nullum. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalosum, lactosum, melibiosum, raffinosum, melez-itosum, amylum solubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum (lente vel exiguum), L-rhamnosum (lente), glycerolum (exiguum, vel lente et exiguum), ribitolum (lente et exiguum, vel nul¬lum), galactitolum, D-mannitolum, D-glucitolum, a¬methyl-D-glucosidum, glucono-6-lactonum, acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum succinicum (exiguum), acidum citricum (exiguum) et inositolum assimilantur, at non L-sorbosum, inulinum, ethanolum, erythritolum, salicinum nec acidum DL-lacticum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 22-23° C. Ad crescentiam acidum thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucle-ico: 44.5 mol% (per HPLC), 42.7 mol% (ex Tm). Ubiquinonum majus: Q-10. Xylosum in cellulis pre-sens.

Holotypus: JCM 8937 (originaliterut NZ-69).

Growth in YM broth: After 5 days at 17° C, the vegetative cells are oval or ellipsoidal, 2.5-4.0 x 3.5-5.5 (Fig. 3A). After one month at 17° C, an imcomplete ring, islets and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is light yellow to apricot, wrinkled, dull, butyrous, and has an undulate margin. Dalmau plate culture on corn meal agar: True mycelia and sometimes large spherical cells measuring up to 13 fxm in diameter are formed (Fig. 3B). Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are round, napiform, turbinate or ampulliform, 2.5-3.0 x 3.0-4.5 µm (Fig. 3C). Fermentation: Negative.

Maximum growth temperature: 21-22° C.
Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Positive (weak).
Liquefaction of gelatin: Negative. Acid production on chalk agar: Negative. Splitting of fat: Negative. Urease: Positive.
Diazonium blue B reaction: Positive.
G + C content of nuclear DNA: 44.7 mol% (by HPLC), 41.0 mol% (from Tm) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-16, which was isolated from a dead leaf of Pseudopanax arboreum infected with sooty moulds at Param Track, Huia, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8933.
B. huiaensis which has mol% G + C of 44.7 seems to have a close relationship to B. hannae which has mol% G + C of 44.5. It has similar characters to the latter species in its assimilation of carbon compounds, requirement of vitamin and maximum growth temperature. However, the difference was found between these two species in the DNA dissimilarity as deduced from DNA-binding experiments (5-7%) (Table 7). Practically, it can be distinguished from the latter species in its assimilation of salicin and cadaverine (Table 8).

In liquid YM post dies 5 ad 17° C, cellulae vegetativae ovoideae vel ellipsoideae, 2.5-4.0 x 3.5-5.5 µm. Pseudomycelium et mycelium formantur. Post unum mensem ad 17° C, anulus imperfecta, insulaeet sedimentum formantur. Cultura in agaro YM, luteola, rugulosa, non-nitida, butyracea et margine undulata. Ballistosporae rotundae, napiformes, tubinatae vel ampulliformes, 2.5-3.0 x 3.0-4.5 µm. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalosum, melibiosum, raffinosum, melezitosum, amylum solubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum (lente), L-rhamnosum (lente), ery thritolum (lente et exiguum, vel nullum), ribitolum (lente et exiguum), galactitolum, D-mannitolum, D-glucitolum (lente vel exiguum), α-methyl-D-glucosidum, salicinum (lente), glucono-ς-lactonum (exiguum), acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum succinicum (exiguum) et inositolum (lente) assimilantur, at non L-sorbosum, lactosum, inulinum, ethanolum, glycerolum, acidum DL-lacticum nec acidum citricum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 21-22° C. Ad crescentiam thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucleico: 44.7 moI% (per HPLC), 41.0 mol% (ex 7rn). Ubiquinonummajus: Q-10. Xylosum in cellulis presens.
Holotypus: JCM 8933 (originaliterutNZ-16).

Growth in YM Broth: After 5 days at 17° C, the vegetative cells are oval or ellipsoidal, 2.5-4.5 x 5.0-6.0 µm, single or in pairs, and septated hyphal cells are present (Fig. 4A). After one month at 17° C, an incomplete ring and a sediment are present.
Growth on YM agar: After one month at 17° C, the streak culture is pinkish-cream, smooth, dull-shining, butyrous, and has an entire margin. Dalmau plate culture on corn meal agar: A few mycelia are formed.
Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are globose, napiform or ampulliform, 2.0-3.5 x 2.5-4.0 pm (Fig. 4B).
Fermentation: Negative.

Maximum growth temperature: 28-29° C. Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Negative.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blueB reaction: Positive.
G + C content of nuclear DNA: 41.7-42.3 mol% (by HPLC), 39.1-41.0 mol% (from 7in) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-44, which was isolated from a dead leaf of Nothofagus fusca at Kaimanawa State Forest Park, Taupo, New Zealand in 1987, is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8934. The other strains NZ-64 and NZ-87 are also deposited in the Japan Collection of Microorganisms, as JCM 8935 and JCM 8938, respectively.
B. mrakii has a close resemblance to B. aumntiaca and B. dendrophila in its biochemical and physiological characters. However, the differences were found between B. mrakii and the latter two species in DNA dissimilarity as deduced from DNA binding experiments (5-13%) (Table 7). Practically, it can be distinguished from the latter two species in its assimilation of melibiose, raffinose and nitrate (Table 8).

In liquid YM post dies 5 ad 17° C, cellulae vegeta-tivae ovoideae vel ellipsoideae, 2.5-4.5 x 5.0-6.0 pm, singulae aut binae. Post unum mensem ad 17° C, anulus imperfecta et sedimentum formantur. Cultura in agaro YM, cremea vel subrosea, glabra, partimdura, butyracea et margine glabra. Ballistosporae rotundae, napiformes vel ampulliformes, 2.0-3.5 x 2.5-4.0 pm. Mycelium formantur. Fermentatio nulla. GIu-cosum, galactosum, L-sorbosum (lente et exiguum, vel nullum), saccharosum, maltosum, cellobiosum, tre-halosum, lactosum (lente vel exiguum, vel nullum), melibiosum, raffinosum, melezitosum, amylum sol¬ubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum, L-rhamnosum, erythritolum (exiguum vel nullum), galactitolum (lente et exiguum, vel nullum), D-mannitolum (lente), D-glucitolum (exiguum vel nul¬lum), a-methyl-D-glucosidum, salicinum, glucono-6-lactonum, acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum DL-lacticum acidum (exiguum, vel lente et exiguum), acidum succinicum, citricum (exiguum) et inositolum assimilantur, at non inulinum, ethanolum, glycerolum nec ribitolum. Kali-um nitricum non assimilatur. Maxima temperatura crescentiae: 28-29° C. Ad crescentiam thiaminum necessarium est. Proportio molaris guanini + cytosi-ni in acido deoxyribonucleico: 41.7-42.3 mol% (per HPLC), 39.1-41.0 mol% (ex 7m). Ubiquinonum majus: Q-10. Xylosum in cellulis presens.
Holotypus: JCM 8934 (originaliter ut NZ-44).

Growth in YM broth: After 5 days at 17° C, the vegetative cells are oval or ellipsoidal, 3.0-4.0 x 4.0-6.0 ftm, single or in pairs (Fig. 5A). After one month at 17° C, a pellicle and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is creamed colored, smooth, dull, soft, and has an entire margin. Dalmau plate culture on corn meal agar: No pseudomycelium and true mycelium are formed. Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are napiform or ampulliform, 1.0-3.0 x 2.5-4.0 µm (Fig. 5B). Fermentation: Negative.

Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Negative.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blue B reaction: Positive.
G + C content of nuclear DNA: 55.4 mol% (by HPLC), 51.1 mol% (from Tm) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-7, which was isolated from a dead leaf of Meliacytis macrophyllus infected with sooty moulds at Param Track, Huia, New Zealand in 1987, is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8932.
B. unica differs from Bullera species with the same G + C content by the DNA dissimilarity as deduced from DNA-binding experiments (4-20%) (Table 5). Practically, B. unica can be discriminated from B. alba, B. armeniaca, B. crocea, B. pseudoalba, B. sinensis and B. variabilis in the lack of assimilation of D-arabinose and D-ribose.

In liquid YM post dies 5 ad 17° C, cellulae veg-etativae ovoideae vel ellipsoid ales, 3.0-4.0 x 4.0-6.0 µm singulae aut binae. Post unum mensem ad 17° C, sedimentum et pellicula formantur. Cultura in agaro YM, cremea, glabra, non-nitida, mollis et margine glabra. Ballistosporae napiformes vel ampulliformes, 1.0-3.0 x 2.5-4.0 µm. Pseudomycelium nullum. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalo-sum, lactosum, melibiosum, raffinosum, melezitosum, amylum solubile, D-xylosum, L-arabinosum, L-rhamnosum, ribitolum (lente), galactitolum (lente et exiguum), D-mannitolum, D-glucitolum, a-methyl-D-glucosidum, salicinum, glucono-5-Iactonum, acidum 2-ketogluconicum, acidum 5-ketogluconicum (exiguum), acidum succinicum (exiguum), acidum cit-ricum (exiguum) et inositolum assimilantur, at non L-sorbosum, inulinum, D-arabinosum, D-ribosum, ethanolum, glycerolum, erythritolum nec acidum DL-Iacticum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 27-28° C. Ad crescentiam thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucleico: 55.4 mol% (per HPLC), 51.1 mol% (ex 7m). Ubiquinonummajus: Q-10. Xylosum in cellulis presens. Holotypus: JCM 8932 (originaliterut NZ-7).