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Bullera huiaensis Hamam. & Nakase 1996

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Hamam. & Nakase
Hamam. & Nakase
1996
284
ICN
Bullera huiaensis Hamam. & Nakase 1996
NZ holotype
species
Bullera huiaensis
Holotypus: JCM 8933 (originaliter ut NZ-16). The strain NZ-16, which was isolated from a dead leaf of Pseudopanax arboreum infected with sooty moulds at Param Track, Huia, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8933.

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huiaensis

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Bullera huiaensis Hamam. & Nakase 1996

Growth in YM broth: After 5 days at 17° C, the vegetative cells are oval or ellipsoidal, 2.5-4.0 x 3.5-5.5 (Fig. 3A). After one month at 17° C, an imcomplete ring, islets and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is light yellow to apricot, wrinkled, dull, butyrous, and has an undulate margin. Dalmau plate culture on corn meal agar: True mycelia and sometimes large spherical cells measuring up to 13 fxm in diameter are formed (Fig. 3B). Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are round, napiform, turbinate or ampulliform, 2.5-3.0 x 3.0-4.5 µm (Fig. 3C). Fermentation: Negative.

Maximum growth temperature: 21-22° C.
Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Positive (weak).
Liquefaction of gelatin: Negative. Acid production on chalk agar: Negative. Splitting of fat: Negative. Urease: Positive.
Diazonium blue B reaction: Positive.
G + C content of nuclear DNA: 44.7 mol% (by HPLC), 41.0 mol% (from Tm) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-16, which was isolated from a dead leaf of Pseudopanax arboreum infected with sooty moulds at Param Track, Huia, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8933.
B. huiaensis which has mol% G + C of 44.7 seems to have a close relationship to B. hannae which has mol% G + C of 44.5. It has similar characters to the latter species in its assimilation of carbon compounds, requirement of vitamin and maximum growth temperature. However, the difference was found between these two species in the DNA dissimilarity as deduced from DNA-binding experiments (5-7%) (Table 7). Practically, it can be distinguished from the latter species in its assimilation of salicin and cadaverine (Table 8).

In liquid YM post dies 5 ad 17° C, cellulae vegetativae ovoideae vel ellipsoideae, 2.5-4.0 x 3.5-5.5 µm. Pseudomycelium et mycelium formantur. Post unum mensem ad 17° C, anulus imperfecta, insulaeet sedimentum formantur. Cultura in agaro YM, luteola, rugulosa, non-nitida, butyracea et margine undulata. Ballistosporae rotundae, napiformes, tubinatae vel ampulliformes, 2.5-3.0 x 3.0-4.5 µm. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalosum, melibiosum, raffinosum, melezitosum, amylum solubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum (lente), L-rhamnosum (lente), ery thritolum (lente et exiguum, vel nullum), ribitolum (lente et exiguum), galactitolum, D-mannitolum, D-glucitolum (lente vel exiguum), α-methyl-D-glucosidum, salicinum (lente), glucono-ς-lactonum (exiguum), acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum succinicum (exiguum) et inositolum (lente) assimilantur, at non L-sorbosum, lactosum, inulinum, ethanolum, glycerolum, acidum DL-lacticum nec acidum citricum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 21-22° C. Ad crescentiam thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucleico: 44.7 moI% (per HPLC), 41.0 mol% (ex 7rn). Ubiquinonummajus: Q-10. Xylosum in cellulis presens.
Holotypus: JCM 8933 (originaliterutNZ-16).

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Bullera huiaensis Hamam. & Nakase 1996
Bullera huiaensis Hamam. & Nakase (1996)
Bullera huiaensis Hamam. & Nakase 1996
Bullera huiaensis Hamam. & Nakase (1996)

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typification
Holotypus: JCM 8933 (originaliter ut NZ-16). The strain NZ-16, which was isolated from a dead leaf of Pseudopanax arboreum infected with sooty moulds at Param Track, Huia, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8933.

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1cb1acaa-36b9-11d5-9548-00d0592d548c
scientific name
Names_Fungi
5 January 2000
31 December 2013
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