Growth in YM broth: After 5 days at 17° C, the vegetative cells are oval or ellipsoidal, 3.0-4.0 x 4.0-6.0 ftm, single or in pairs (Fig. 5A). After one month at 17° C, a pellicle and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is creamed colored, smooth, dull, soft, and has an entire margin. Dalmau plate culture on corn meal agar: No pseudomycelium and true mycelium are formed. Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are napiform or ampulliform, 1.0-3.0 x 2.5-4.0 µm (Fig. 5B). Fermentation: Negative.

Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative.
Production of starch-like substances: Negative.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blue B reaction: Positive.
G + C content of nuclear DNA: 55.4 mol% (by HPLC), 51.1 mol% (from Tm) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-7, which was isolated from a dead leaf of Meliacytis macrophyllus infected with sooty moulds at Param Track, Huia, New Zealand in 1987, is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8932.
B. unica differs from Bullera species with the same G + C content by the DNA dissimilarity as deduced from DNA-binding experiments (4-20%) (Table 5). Practically, B. unica can be discriminated from B. alba, B. armeniaca, B. crocea, B. pseudoalba, B. sinensis and B. variabilis in the lack of assimilation of D-arabinose and D-ribose.

In liquid YM post dies 5 ad 17° C, cellulae veg-etativae ovoideae vel ellipsoid ales, 3.0-4.0 x 4.0-6.0 µm singulae aut binae. Post unum mensem ad 17° C, sedimentum et pellicula formantur. Cultura in agaro YM, cremea, glabra, non-nitida, mollis et margine glabra. Ballistosporae napiformes vel ampulliformes, 1.0-3.0 x 2.5-4.0 µm. Pseudomycelium nullum. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalo-sum, lactosum, melibiosum, raffinosum, melezitosum, amylum solubile, D-xylosum, L-arabinosum, L-rhamnosum, ribitolum (lente), galactitolum (lente et exiguum), D-mannitolum, D-glucitolum, a-methyl-D-glucosidum, salicinum, glucono-5-Iactonum, acidum 2-ketogluconicum, acidum 5-ketogluconicum (exiguum), acidum succinicum (exiguum), acidum cit-ricum (exiguum) et inositolum assimilantur, at non L-sorbosum, inulinum, D-arabinosum, D-ribosum, ethanolum, glycerolum, erythritolum nec acidum DL-Iacticum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 27-28° C. Ad crescentiam thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucleico: 55.4 mol% (per HPLC), 51.1 mol% (ex 7m). Ubiquinonummajus: Q-10. Xylosum in cellulis presens. Holotypus: JCM 8932 (originaliterut NZ-7).