Bullera hannae Hamam. & Nakase 1996
Details
Nomenclature
Classification
Associations
Descriptions
Bullera hannae Hamam. & Nakase 1996
Growth in YM broth: After 5 days at 17° C, the vegetative cells are short oval to oval, sometimes ellipsoidal, 2.5^.0 x 4.0-6.0 µm (Fig. 2A). After one month at 17° C, a ring, islets and a sediment are present. Growth on YM agar: After one month at 17° C, the streak culture is yellowish white to light yellow, smooth, shining, soft, and has an entire margin. Dalmau plate culture on corn meal agar: Mycelium and pseudomycelium are not formed. Ballistospores: Ballistospores are produced on corn meal agar and YM agar. They are round, napiform, turbinate or ampulliform, 2.2-3.2 x 2.0-4.0 ^m (Fig. 2B). Fermentation: Negative.
Maximum growth temperature: 22-23° C. Vitamins required: Thiamine. Growth on 50% (w/w) glucose-yeast extract agar: Negative. Production of starch-like substances: Negative.
Liquefaction of gelatin: Negative.
Acid production on chalk agar: Negative.
Splitting of fat: Negative.
Urease: Positive.
Diazonium blueB reaction: Positive.
G + C content of nuclear DNA: 44.5 mol% (by HPLC), 42.7 mol% (from 7m) (Table 3).
Major ubiquinone: Q-10 (Table 4).
Xylose in the cells: Present.
Strains examined: The strain NZ-69, which was isolated from a dead leaf of Pseudowintera colonata infected with sooty moulds at Kaiwanawa State Forest, Paupo, New Zealand in 1987 is the type strain of the species. This strain is deposited at the Japan Collection of Microorganisms, Wako, Saitama, as JCM 8937.
B. hannae has similar assimilation pattern to B. mrakii and has mol% G + C of 44.5 which is the similar to B. huiaensis. However, differences were found between B. hannae and the latter two species in the DNA dissimilarity as deduced from DNA-binding experiments (5-10%) (Table 7). It clearly indicates that B. hannae is a different species from B. huiaensis and B. mrakii.
Practically, B. hannae can be discriminated from B. huiaensis in the lack of assimilation of cadaverine and from B. mrakii in the lack of assimilation of salicin (Table 8)
In liquid YM post dies 5 ad -17° C, cellulae vegetativae subovoideae, ovoideae vel ellipsoideae, 2.5-4.0 x 4.0-6.0 µm. Post unum mensem ad 17° C, anulus, insulae et sedimentum formantur. Cultura in agaro YM, flavo-albida aut subflava, glabra, nitida, mollis et margine glabra. Ballistosporae rotundae, ampulliformes aut turbinatae, 2.2-3.2 x1 2.0-4.0 µm. Pseudomycelium nullum. Fermentatio nulla. Glucosum, galactosum, saccharosum, maltosum, cellobiosum, trehalosum, lactosum, melibiosum, raffinosum, melez-itosum, amylum solubile, D-xylosum, L-arabinosum, D-arabinosum, D-ribosum (lente vel exiguum), L-rhamnosum (lente), glycerolum (exiguum, vel lente et exiguum), ribitolum (lente et exiguum, vel nul¬lum), galactitolum, D-mannitolum, D-glucitolum, a¬methyl-D-glucosidum, glucono-6-lactonum, acidum 2-ketogluconicum, acidum 5-ketogluconicum, acidum succinicum (exiguum), acidum citricum (exiguum) et inositolum assimilantur, at non L-sorbosum, inulinum, ethanolum, erythritolum, salicinum nec acidum DL-lacticum. Kalium nitricum non assimilatur. Maxima temperatura crescentiae: 22-23° C. Ad crescentiam acidum thiaminum necessarium est. Proportio molaris guanini + cytosini in acido deoxyribonucle-ico: 44.5 mol% (per HPLC), 42.7 mol% (ex Tm). Ubiquinonum majus: Q-10. Xylosum in cellulis pre-sens.
Holotypus: JCM 8937 (originaliterut NZ-69).