To better understand the relationship between Podospora anserina and its closely related species, we re-analysed the published sequence data generated by Boucher et al. (2017), including ITS sequences and sequences of three other intergenic loci from different chromosomes (Rchr3, Rchr4 and Rchr6). Single gene trees were constructed based on each locus. ITS failed to distinguish Pod. anserina from Pod. pauciseta, Pod. bellae-mahoneyi and Pod. pseudocomata (Fig. 6A). In contrast, Rchr3 (Fig. 6B), Rchr4 (Fig. 6C) and Rchr6 (Fig. 6D) differentiated all seven species which were accepted by Boucher et al. (2017), and Rchr3 even recognised two subclades within the P. anserina clade.