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Dingley, J.M. 1972: Some foliicolous ascomycetes on ferns in Australia and New Zealand. The genera Rhagadolobium P. Henn. & Lind. and Lauterobachiella Theiss. & Syd. New Zealand Journal of Botany 10(1): 74-86.

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Dingley, J.M. 1972: Some foliicolous ascomycetes on ferns in Australia and New Zealand. The genera Rhagadolobium P. Henn. & Lind. and Lauterobachiella Theiss. & Syd. New Zealand Journal of Botany 10(1): 74-86.
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on Dicksonia antarctica Labill. Australia: Victoria, Marywille, SIVI/41 E. Fisher (K) (MEL) on D. lanata Col. New Zealand: Auckl ' and, Dargaville, Trounson Kauri Park, 15/1X/67 J.M.D.. (PDD 26077); Waipoua, J.M.D. 16/1X/67 (PDD 26079). on D. squarrosa (Forst. I.) Swartz New Zealand: Auckland, Waitakere Ra., Sharp's Bush J.M.D. 6/X165 (PDD 24774) Type- collection: J.M.D. 30/VIII/69 (PDD 28435) Waitakere Ra., Huia, W P. J. Brook 5 IX 69 (PDD 26652); Hauhaungaroa Ra Saddle 1129/IX 69 (PDD 26653); Westland, Harihari, Little Wanganui R April 1963,S. J. Hughes and J.M.D. (PDD 25995).
Ascomata hypophyllous, angular, sometimes globose 1-3 mm diam., superficial, crustose, grey-black; at first subcuticular, mycelium, penetrating tissues of the leaf through stomata, 20-25 u thick, to 50 um thick at loculi. Outer wall of radially arranged, darkly pigmented, agglutinated mycelium 3-5 u diam. Hypostroma, hyphae at first lightly pigmented becoming darker 5-6um diam. Loculi 60-120um diam. linear, 0.51.0 mm long, covered by pigmented agglutinated hyphae which at maturity cracks longitudinally in centre of loculus. Hymenium of asci only, small groups of the pigmented agglutinated cells adhering to hymenium. Asci elliptical or clavate 30-47 X 8-12 u, bitunicate, eight spored, ascospores biseriately arranged in asci. Ascospores one septate, elliptical or pyriform, unevenly divided by septa, 8-12.5 X 2.5-3.5um smooth, lightly pigmented when mature.
Ascomata in hypophyllo, angularia, interdurn globosa 1-3mm diam.; supra nigrescentia, 20-25um,, densa 50um densa apud leculos; pariete exteriore, hypharum radiatim dispositarum 5-6um diam. Loculi 60-120um diam. lineares, 0.5-1mm longi. Hymenium modo ascorum. Asci elliptici vel clavati 30-47 x 8-12um, bitunicati, sporis octo, biseriatim dispositis. Ascosporae una septata, elliptica vel pyriformis, impariter divisa septis 8-12.5 X 23-3.5um, levis modice colorata.

In the herbarium, Royal Botanic Gardens Kew there file as Dothidea filicina (Fr.)Fckl., a collection N.Z. Colenso, ex Herb Berkeley. The host although unlabelled is typical of Dicksonia rosa. Berkeley (1855) records Dothidea filicina Mont. mss; -a~, 2~n the underside of fern leaves, Colenso. He noted Dr Montagne's mens are from Otaheite (Tahiti) on some species of Adiantum and forms occur in Ceylon of which a description will shortly appear in the Journal of Botany. In that the asci are short, obovate, and the obovate, uniseptate 1/2000 of an inch long. 1 have seen no fruit in New Zealand specimens but they have the same brittle stroma and rhytismoid aspect. Berkeley (1854) described Dothidea filicina Mont. mss var. nervisequia from Ceylon (G. H. K. Thwaites). He noted that this species differed in appearance from D.,pteridis Fr. (syn. Cryptomycina pteridis (Fr.) Hohnel) and was merely a form of Dr Montagne's species on Adiantum from Tahiti. He also noted that the same species occurred in New Zealand. Among species disposed by Muller and Arx under Rhagadolobium is R. nervisequium (Berk.) v. Arx based on the Ceylon material. Montagne does not appear to have published the species Dothidea filicina. Dothidea filicina Fr. refers to a different species. In the Dothidea filicina folder at Kew there are both the Ceylon collection and the collection from New Zealand. The New Zealand species on Dicksonia appears to be distinct from the Ceylon material in that loculi are larger and ascospores are smaller. Both species are characteristic of Lauterbachiella.

On Dicksonia squarrosa; Auckland, Waitakere Ra., Sharp's Bush, J.M.D. 6/X/1965 (PDD 24774).
on Cyathea gigantea (Wall. ex Hook.) HoItt. (syn. Alsophilae gigantea Wall. ex Hook.) Ceylon: Central Province, Galagaira, May, 1858 on Cyathea rebeccae (v. Miffier) Domin. (syn. Alsophila rebeccae v. Muller) Australia: North Queensland, F. v. Muller (K) (type collection of Asterina alsophilae Cke. & Mass.) on unknown host Australia: Queensland, Russell R. (K) on Cyathea smithii Hook f. New Zealand: Auckland, L. Waikaremoana, J. M. Dingley Nov. 1956 (PDD 16077) There is a need for further collections of this species; in the three Kew collections spores are immature. In the New Zealand collection they are 9-12 X 3-3.5 g, similar to those given for the species by Theissen and Sydow (1915). The species differs from L. dicksoniafolia in that loculi may be annular and pseudoparaphyses are present in hymenium. The conidial stage Queenslandia pulchella Batista & Maia in Batista and Ciferri (1959) appears to be a misinterpretation of immature tissues in developing ascogenous loculi.

Ascomata on upper and lower surfaces of fern leaves, more or less circular, 13-3 mm diam., when mature entirely superficial, black, crustose, at first subcuticular, mycelium of ascomata only in contact with leaf tissue through stomata; 15-20um thick, 80 A at loculi. Outer wall of radially arranged, pigmented and agglutinated mycelium. Hypostroma of pigmented and thickened hyphae. Loculi annular or linear 60-80um diam., up to 0.5 mm Ion., covered by agglutinated mycelium which at maturity cracks and exposes hymenium. Hymenium of closely compacted asci, pseudoparaphyses absent. Asci elliptical or clavate 50-6011 X 5-8 um bitunicate, 8 spored, ascospores biseriately or uniseriately arranged. Ascospores one septate, elliptical, 9-12 X 3-3.5 um hyaline.

Ceylon, Australia, New Zealand.

On living leaves of Cyathea spp. TYPE: On Alsophila gigantea Wall. ex Hook., Ceylon, Central Province, Galagaira, May, 1858 (Thwaites, 453)

on Cyathea sp. Philippine Is.: Mt Maquiling, Luzon, April, 1915 leg. Baker, 3841. (Fungi Malayana 282) (K) on C. caudata (J. Smith) Copeland: Philippine Is.: Mt Maquiling, Luzon June, 1914, leg. Baker (Fungi Malayana 569) (K) on C. dealbata (Forst, f.) Swartz New Zealand: Auckland; Hunua Ra., L. B. Cranwell -1X/32 (PDD 19430) (PDD 19432); LM.D. -1111158 (PDD 19431); Waitakere Ra., Swanson, LM.D. -/1V/54 (PDD 19808); Waitakere Ra., Waiatarua, J.M.D. -/V1/58 (PDD 19433); Waltakere Ra., Waiatarua, Parker's Rd., J.M.D. 28/VII/69 (PDD 26565); Waitakere Ra., Henderson Valley, Sharp's Bush, J.M.D. 30/VIII/69 (PDD 28417); Waitakere Ra., Huia, Karamatua St., P. J. Brook, 5/1X/68 (PDD 28104); Manurewa, J.D.M. Il/VIII/69 (PDD26564); Little Barrier Isl. J.M.D. -/X/45 (PDD 19035); Thames, Kaueranga R., -/VIII/64 (PDD 23725), J.M.D. 2/VIII/70 (PDD 28542); Coromandel, Whitianga Rd., Kauri Grove, J.M.D. 22/XII/69 (PDD 28139) (PDD 28410); Wellington, Wairarapa, L. Pounui, L. B. Moore -/IX/60 (PIDD 19484); Mt Pihinga, near Tokaanu, J.M.D. 27/1X/69 (PM 28416) (PDD 28418).
Ascomata solitary or in clusters 1-2 mm diam., hypophyllous, superficial 200-350 um thick, grey black, concentric or cupshaped when mature, margins inrolled, often splitting into a number of irregular lobes, centrally attached to leaf, associated with grey brown angular necrotic lesions 2-3 mm diam. Ascomata pseudoparenchymatous, at first subcuticular then erumpent, outer cells radially arranged, pigmented and agglutinated; hypostroma prosenchymatous, light coloured, agglutinated, continuous with intercellular mycelium in leaf. Loculi annular embedded in lobes surrounding a sterile disc; at maturity annular cracks are formed in centre of loculi; margins becoming inrolled to expose the disclike hymenium. Hymenium 60-80 g, composed of asci and pseudoparaphyses; small clusters of dark cells of outer cover often adhering to it. Asci bitunicate, elliptical or clavate, eight spored, ascospores obliquely uniseriate, often biseriate at apex. Pseudoparaphyses filamentous, septate, terminal cells swollen, club shaped, up to 5 11 diam. Ascospores one septate, elliptical, occasionally pyriform, slightly pigmented
Philippine Is., New Zealand.
Hypophyllous on leaves Of Cyathea spp.

Part of the type of R. bakerianum labelled Fungi Malayana CF. Baker 282'' in the Kew herbarium has been examined. Saccardo (1916) gives the type locality as Mt Maquiling, Philippines Is., April 1915 (Baker 3841); also in Kew but filed under Pachypatella alsophilae there is a collection labelled Fungi Malayana CF. Baker 569, Pachypatella alsophilae (Rac.) Theiss. & Syd. on Cyathea caudata, Mt Maquiling summit, near Los Banos, Prov. Laguna, June, 1914, Det. Sydew. Both these collections appear to be on the same host and from the same place but collected at different times. Examination of this material has shown the two to be collections of the same fungus. Since Sydow (1915) listed this species as Pachypatella alsophilae it is no wonder that he (Sydow, 1922) regarded Rhagadolobium bakerianum as a synonym of Pachypatella alsophilae.

Unfortunately the type of P. alsophilae has not been examined but in the species description no mention is made of the pseudoparaphyses which are always present in the hymenium of Rhagadolobium bakerianum. Therefore Rhagadolobium bakerianum is regarded as a distinct species; no doubt Pachypatella alsophilae is congeneric with it.

R. bakerianum differs from R. hemiteliae in that fructifications are larger, the ascomata is always well developed, and each loculus is formed in a separate tubercle; ascospores are larger and pseudoparaphyses are always present in the hymenium

On Cyathea sp. Mt Maquiling, Luzon, Philippine Is., April 1915 (Baker, 3841)
Ascomata 0.5-1 mm diam. epiphyllous or hypophyllous, shieldlike, more or less orbicular, superficial, centrally attached to leaf, brown black crustose, associated with small angular necrotic leaf lesions, 1-2 mm. diam, Ascomata at first subcuticular, then superficial, 45-75 um thick, pseudoparenchymatous; outer hyphae 3-3.5 um diam., agglutinated, pigmented brown black; hypostroma of lighter coloured. thin walled mycelium, more or less pseudo parenchymatous, cells 2-3.5 X 5-6 u continuous with intercellular mycelium in leaf tissue; uni- or multilocular. Loculi orbicular rarely obpyriform 40-400 g diam. when immature covered by layer of dark coloured agglutinated mycelium, which at maturity cracks and becomes inrolled to expose hymenium. Hymenium disclike, 60-80 g thick composed only of asci. Asci bitunicate 42-84 x 12-15 u, eight spored, ascospores obliquely uniseriate sometimes biseriate at apex. Pseudoparaphyses diffluent absent in mature ascomata. Ascopores oneseptate, elliptical or pyriform, 9.5-13.5 X 3.5-5 u unevenly divided by septa, smooth, lightly pigmented brown when mature.

Epiphyllous or hypophyllous on living leaves of Cyathea spp.

on Rhagadolobium bakerianum Auckland ' Waitakere Ra., Sharp's Bush, LM.D. 30/VIII/69 (PDD 28170); Huia, P. J. Brook, 5/1X/69 (PDD 28419); Waiatarua, J.M.D. 181111/70 (PDD 28420); Coromandel, Whitianga Rd, Kauri Grove, J.M.D. 18/III/70(PDD 28458).
Mycelium composed of repent hypha, brown with thickened echinulate walls, septate, sparsely branched, 1-3 um diam. ramifying through tissues of ascomata of Rhagadolobium often extending as a superficial layer on fern frond. Conidiophores in dense clusters formed singly at right angles from basal mycelium, 400-800 um long, base rarely swollen, 2-2.5 u diam. rarely branched, sparsely septate, at first thin walled, olivaceous then becoming brown and with roughened walls. Conidia produced singly at tip as blown-out ends, continuously, in acropetal succession leaving a narrow geniculate scar. Conidia one to three septate, elliptical or pyriform, not constricted at septa, olivaceous or fuscous_ finely echinulate, basal end tapering to a broad truncate basal scar 6-13.5 X 3-4 um.
On ascomata of Rhagadolobium bakerianum
Mycelium hyphis compositum, sparsim ramosis, septatis brunneis; paretibus inspissatia 1-3um diam, conidiophora in fasciculis densis, singulatim producta angulo recto e mycelio basali, 2-2.5um densa, 400-800um longa, sparsim septata, raro ramosa, olivacea, parietibus scabris apud apicem cicatricibus angustis et geniculatis. Conidia singulatim apud apicern producta in serie acropeta. Conidia unum interdurn tria, septata elliptica vel pyriformia, non apud septa constricta, olivacea, subtiliter echinulata 6-13.5 x 3-4um
In a number of collections of R. bakerianum ascomata are completely smothered with dark brown floccose mycelium. Microscopic examination has shown that this mycelium belongs to a hyphomycete parasitising the ascomata. Hohnel (1909) described a Cladosporium-like hyphomycete as a conidial form of Discodothis filicum v. Hohnel. Muller and v. Arx: (1962) included D. filicum in Rhagadolobium; they commented on and illustrated a Cladosporium sp. attacking the ascomata as a hyperparasite. New Zealand material of infected ascomata of Rhagadolobium bakerianum was forwarded to Mr F. C. Deighton at the Commonwealth Mycological Institute, Kew. He suggested that the New Zealand hyperparasite was probably the same as the fungus illustrated by v. Arx in Muller and Arx, (1962) but that it could not be classified under Cladosporium. Dr M. B. Ellis at the Institute suggested that it best be classified under Veronaea Cifferri & Montemartini. Originally this =us was erected to include a Cladosporium- like fungus which grew on olive waste in Italy. Papendorf (1969) suggested that the genus was synonymous with. Sympodina Subramanian & Lodha and described a new species in Veronaea, V. simplex, from soil. The species, present in New Zealand on Rhagadolobium, is similar to the three species described in Veronaea but differs in that conidiophores are simple and rarely branched and conidia are more sparsely produced; they may be one- or three- septate; therefore this New Zealand species is described as new.
On Rhagadolobium bakerianum; Auckland, Waitakere Ra., Sharp's Bush, J.M.D. 30/viii/69 (PDD 28170).

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Rhagadolobium species

1
Ascomata up to 1 mm diam., ascospores 4-5 um diam.
Ascomata over 1 mm diam., ascopsores up to 6 um diam.

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1cb0df2a-36b9-11d5-9548-00d0592d548c
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3 September 2004
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