D. ananatis is a Gram-negative, rod-shaped, motile bacterium measuring 2.91±0.02 µm in length, with peritrichous flagella. It is fermentative and grows optimally at 28 °C on nutrient agar medium supplemented with 0.4% glucose and on King’s B medium, forming milky white colonies within 24 h. This bacterium forms pits on the CVP medium within 24–48 h [33] and causes maceration of potato tubers, taro slices and pineapple leaves. D. ananatis sp. nov. is catalase positive, oxidase negative, pectinolytic and positive for nitrate reduction. D. ananatis sp. nov. is able to utilize sucrose, d-raffinose, d-melibiose, β-methyl-d-glucoside, d-salicin, N-acetyl-d-glucosamine, d-glucose, d-mannose, d-fructose, d-galactose, d-sorbitol, d-mannitol, myo-inositol, glycerol, d-glucose-6-PO4, d-fructose-6-PO4, d-aspartic acid, l-aspartic acid, l-glutamic acid, l-serine, pectin, d-galacturonic acid, l-galactonic acid lactone, d-gluconic acid, d-glucuronic acid, methyl pyruvate, l-lactic acid, citric acid, d-malic acid, l-malic acid, bromo-succinic acid, acetic acid and formic acid as carbon sources. It shows inhibition at pH 5, or in the presence of 8% NaCl, d-serine, minocycline, nalidixic acid, potassium tellurite, aztreonam and sodium bromate. The type strain is unable to utilize the following carbon sources: dextrin, stachyose, N-acetyl neuraminic acid, d-serine, quinic acid, p-hydroxy-phenylacetic acid, d-lactic acid methyl ester, Tween 40, ϒ-amino-butyric acid, α-hydroxy-butyric acid, β-hydroxy-D, l-butyric acid, α-keto-butyric acid and propionic acid. Enzymatic reactions tested with the API ZYM kit give positive results for alkaline phosphatase, esterase, leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase and β-glucosidase. The type strain is able to utilize citrate, produces indole and acetoin and is able to hydrolyse aesculin; it is negative for urease. Assays for antibiotic susceptibility showed that D. ananatis sp. nov. A5410T is sensitive to 13 antibiotics including trimethoprim, kanamycin, bacitracin, cephalexin, hygromycin B, vancomycin, cefotaxime, tetracycline, spectinomycin, chloramphenicol, carbenicillin, penicillin and gentamicin. The highest sensitivity was observed for cefotaxime, carbenicillin and cefalexins. The 16S rRNA gene sequence is available in GenBank (OR271567). The type strain A5410T genome from a previous study can be found under CP040816 (BioProject PRJNA544764, BioSample SAMN11855518). The genomes sequenced in this study have been submitted to the database with the following accession numbers: CP040816, CP146223, CP146227, CP146228, CP146224, CP146225 and CP146226.

The D. ananatis sp. nov. type strain is A5410T (=ICMP 25020T=LMG 33197T) isolated from pineapple in Hawaii. Its genome size is 4.77 Mbp with a G+C content of 53.5 mol% based on the genome sequence. Other isolates of this species were also obtained from pineapple in Hawaii (A5391, A6136, A6137 and A5611), and two strains, CFBP 1272 and CFBP 1278, were isolated from pineapple in Malaysia.