


Lentinellus marginatus Segedin 1996

Details
Nomenclature
Classification
Descriptions
Lentinellus marginatus Segedin 1996
Spores 4.0-5.5 x 3.5 4.5 (4.9 x 4.0) µm, Q = 1.2, subglobose, hyaline, wall strongly amyloid, with short peg-like ornamentation and distinct apiculus. Basidia 20 - 30 x 6 µm, clavate, mostly 4-spored, sometimes 2-spored, each with clamp connection on basal septum. Leptocystidia plentiful on all parts of the lamellae, mostly subulate, narrowly to broadly fusiform or narrowly lageniform, 30-60 x 3 - 12 µm, thin-walled, usually without contents and each with a clamp connection at the base. A few clavate to lanceolate pseudocystidia also present. Trama of more or less parallel, thin-walled, clamped, descending hyphae, up to 3 µm diam. Many parallel, oleiferous hyphae present, 3-5 µm diam., becoming contorted, with some empty segments and slight epimembranal incrustation, near the very narrow, cellular sub-hymenium. No thick-walled hyphae in trama. Trama ochraceous in KOH, negative in Melzer's reagent. Context of interwoven hyphae, relatively thin-walled, up to 6 µm diam., with plentiful oleiferous hyphae concentrated particularly near the pileipellis. Oleiferous hyphae up to 7 µm diam., often much contorted and with swellings up to 14 µm diam. Skeletal hyphae absent. Middle region of context dextrinoid but colour fades fairly quickly. Pileipellis of repent, interwoven, yellowish-brown, thin-walled hyphae, 2 - 3 µm diam., with many interspersed oleiferous hyphae, the ends of which protrude as numerous pseudocystidia, especially near the stipe attachment. Pseudocystidia clavate, lageniform or lanceolate, 50- 90 x 12 - 13 µm, walls only slightly thickened at first but those towards the stipe becoming thick-walled. Sections through the margin of the pileus show clusters of clavate cells 20-30 x 5 - 7µm, intermingled with basidia and pseudocystidia. The apices of these clavate cells become converted into thick-walled chlamydospores, subpyriform when first produced but after secession becoming subspherical, 5.5-8.0 x 4.5-6.5 (7.15 x 5.8) µm, Q = 1.2, with walls up to 3 µm thick, inamyloid, not dextrinoid. Stipe of more or less parallel hyphae up to 3 µm diam. with slightly thickened walls, together with oleiferous hyphae of slightly larger diameter and some thick-walled hyphae up to 6 µm diam., with walls up to 2 µm. Surface tomentum of stipe of tangled, hyaline, branched, narrow (up to 2 µm) clamped hyphae, mixed with oleiferous hyphal endings and a few fusiform leptocystidia; chlamydospores, detached from the margin of the pileus, are commonly found adhering to the tomentum hyphae, but never seen with a germ tube. The tough, bristly hairs up to 2 mm long that protrude through the tomentum consist of narrow (1-2 µm diam.), slightly thick-walled, brown, agglutinated hyphae, with occasional clamp connections.
ETYMOLOGY: Referring to the chlamydospores on the margin of pileus and the white edge of the lamellae.
The production of chlamydospores on the margin of the cap is a very distinctive character in Lentinellus marginatus. L. cochleatus seems to be the only other species reported to produce chlamydospores but they are described as being either on the stipe (Miller & Stewart 1971) or formed interstitially beneath the pileipellis (Breitenbach & Kranzlin 1991). Also, the basidiomata of L. cochleatus are different in form, with well developed, long stipes in tightly fused caespitose clusters (Miller & Stewart 1971).