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Simmons, E.G. 2001: Perfect states of Stemphylium - IV. Harvard Papers in Botany 6(1): 199-208.

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Simmons, E.G. 2001: Perfect states of Stemphylium - IV. Harvard Papers in Botany 6(1): 199-208.
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Ascomata of P. eturmiuna develop abundantly within and on PCA and V-8 agar substrates, almost to the point of crowding. On PCA, mature ascomata are dark, subsphaeroid, ca. 200-300 µm diam., with a small ostiolar beak and numerous dark, flexible hyphae that arise in surface cells of the ascoma. Asci are long ovoid or oblong, straight or curved, ca. 140-150 x 25-30 µm, usually 8-spored. Ascospores are ca. 17-18 x 7-8 µm, ellipsoid or oblong when the first transeptum forms. Ascospores develop through narrow ellipsoid, to broader ellipsoid, then to a minor extent to broad-oblong with broadly rounded end cells. In any examination of ascomata at 3-4 weeks, broadly ellipsoid ascospores ca. 28-32(-36) x 11-13 µm dominate the spore population; oblong spores with broadly rounded ends and ca. 28 x 10-11 µm, apparently derived by shortening from the ellipsoid stage, are not common. Ascospores have 7-8 transepta and 1(-2) longitudinal or oblique septa; they always are moderately to markedly constricted at the three primary transepta, especially during intermediate stages of development. Color is a dilute dull to medium yellowish tan.
The anamorph Stemphylium eturmiunum grows well and sporulates abundantly on PCA, Hay, and V8 agar substrates, predominantly on short branches of erect or procumbent hyphae that arise singly or in fascicles from the substrate. Aerial axis hyphae commonly are 1 mm or more in length and bear very large numbers of short conidiogenous branches ca. 10-40 x 6 µm with a tip cell only slightly wider. Juvenile -conidia are oblong and ca. 18 x 7 µm when the first transeptum forms. The great majority of conidia mature as broadly ovoid or ellipsoid units, ca. 25-27 x 12 µm, with three transverse or oblique septa and one .longitudinal or oblique septum in each of the transverse divisions. A minor portion of the conidium population matures as broadly oblong units, ca. 27-32 x 13-16 µm, with 3-5 transepta and 1(-2) longitudinal or oblique septa in all transverse divisions. Conidium color is medium brown with septa even darker; wall ornamentation is punctulate to punctate overall.
Ex - cultura in agaro PCA descripta. Ascomata atrobrunnea, subglobosa, ca. 200-300 µm diam., pariete tenui, rostellata. Asci longe ovoidei vel oblongi, aequilateri vel curvati, ca. 140-18O x 25-30 µm, plerumque octospori. Ascosporae ellipsoideae vel oblongae tam anguste ellipsoideae tam late ellipsoideae tam aliquando late oblongae, extremitatibus late rotundatis; plerumque ellipsoideae ca. 28-32(-36) x 11-13 µm, infrequenter oblongae ca. 28 x 10-11 µm; transverse 7-8 septatae, longitudinaliter 1(-2) septatae, in transeptis primariis tribus constrictis, dilute luteobrunneolae. Conidiophora plerumque lateraliter, numerosa, 10-40 x 6 µm, in hyphis longis, erectiusculis, arcuatis, solitariis vel fasciculatis 1 mm x, 8 µm. Conidia oblonga, late ovoidea vel ellipsoidea, plerumque ca. 25-27 x 12 ,aria, transverse 3 septata, longitudinaliter vel oblique 1 septata; modice brunnea, septis conspicue atrobrunneis; punctulata vel punctata.
Etymology: Latin, e + turma + una, one (representative) of a (species) group.
TYPE: NEW ZEALAND. Levin: on fruit of Lycopersicon esculentum Mill., 1969, N. Z. Dept: Agriculture, G. F. Laundon LEV 3417. Dried culture of ex-Conidum isolate E. G. Simmons 29-099 (Holotype: BPI 747304!). Fig. 4. Anamorphosis: Stemphylium eturmiunum E. G. Simmons, anam. nov.
Ascomata of P. sedicola develop in large numbers within all three agar substrates used. They contain asci with immature and a few fully mature ascospores within ca. 3 wk when they develop on alfalfa stems placed on PCA colonies; 4 wk or longer apparently is required for maturation of large numbers of ascospores under standard conditions of culture. Mature ascomata are dark, thin-walled, subsphaeroid, ca. 350 µm diam., with a small apical, ostiolar beak. Asci are elliptical or oblong, ca. 150-170 x 30 µm, usually 8-spored. Ascospores are ca. 30 x 6 pin, strongly fusiform, when the first transverse septum forms. Ascospores remain fusiform as length and width increase to ca. 35-40 x 8-10 µm; then become broader and shorter (broadly elliptical) as they reach maximum septation at ca. 28-32 x 10-12 µm, with 7 transepta and 1-2 longitudinal or oblique septa in most or all transverse divisions. It is emphasized that developing ascospores before full septation-including minimally septate juveniles-have lengths in a 30-40 µm range; full development of the ascospore, at ca. 28-30 x 12 µm, is a process of contraction in length as the spore increases in width. Ascospore color develops from a juvenile subhyaline. to a mature dull, dilute brown. Ascospores are strongly constricted at all three primary transepta throughout the fusiform stages; constriction remains evident but less striking in the fully developed ascospore.
The anamorph Stemphylium sedicola grows well and sporulates very abundantly on Hay and V-8 agars. On PCA, however, solitary conidia are produced almost entirely on numerous short branches of long, sturdy, erect or arching hyphae that arise singly or in fascicles in concentric rings of growth; the axis hyphae commonly are as long as 1 mm, the conidiogenous branches 32-90 x 5 µm with a slightly wider tip cell. Juvenile conidia are ovoid and ca. 8-14 x 7-8 µm when the first transeptum forms; they are punctulate from the beginning of development. Conidia become broadly ellipsoid or oblong with broadly rounded base and apex; 30-35 x 18-20 µm, with 2-3 complete primary and a few partial transverse or oblique septa, 1-3 longitudinal or oblique septa inserted somewhat irregularly within transverse divisions. Conidia usually are constricted at 1-2 primary transepta, yellow-tan to medium yellow-brown, with walls smooth or usually punctate roughened overall.
Ex cultiura in agaro PCA descripta. Ascomata atrobrunnea, subglobosa, ca. 350 µm diam., pariete tenui, rostellata. Asci ellipsoidei vel oblongi, ca. 150-170 x 30 µm, plerumque octospori. Ascosporae valde fusoideae tum anguste ellipsoideae, ca. 35-40 x 8-10 µm, denique late ellipsoideae, ca. 28-32 x 10-12 µm, transverse 7-septatae, fortiter vel moderate constrictae, longitudinaliter vel oblique 1-2-septatae; initio subhyalinae, denique dilute obscure brunneolae. Conidiophora numerosa, 32-90 x 5 µm in hyphis longis, erectiusculis, arcuatis, solitariis vel fasciculatis, ad ca. 1 mm x 5 µm. Conidia ovoidea tum late ellipsoidea vel oblonga, µmdo et apice late rotundatis, 30-35 x 18-20 µm, transverse 2-3 omnino et aliquot imperfecte septata, longitudinaliter 1-3 irregulariter septata, plerumque in 1-2 transeptis primariis constrictis, dilute vel modice luteobrunneola, parietibus laevibus vel punctatis.
Etymology: Latin, Sedum + -cola, inhabiting a Sedum species.
TYPE: NEW ZEALAND. Auckland, on leaf lesions of Sedum spectabile Boreau, 18 Feb. 2000, N. Z. Ministry of Agriculture and Fisheries LYN 124. Dried culture of ex-conidium isolate E. G. Simmons 48-095 (Holotype: BPI 747302!). Fig. 2. Anamorphosis: Stemphylium sedicola E. G. Simmons, anam. nov.

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29 March 2004
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