Our specimens from New Zealand fit well the description of L. investita, although the ascospores in PDD 110876 are more tapering towards the ends. This collection was isolated into axenic culture, which is, unfortunately, no longer available. The fungus formed in vitro a phaeoisaria-like asexual morph, which is consistent with asexual morphs observed in L. sulcata and Lentomitella sp. The DNA extraction from herbarium material was not successful. Lentomitella investita, L. vestita and L. cirrhosa were formerly treated as conspecific (von Arx 1952, R eblov a 2006). Based on molecular evidence the two latter are accepted as separate species in our study. Although no DNA sequence data of L. investita are available, given the size of asci and ascospores, the species is intermediate between L. vestita and L. cirrhosa and therefore regarded as distinct. Lentomitella cirrhosa differs from L. investita by longer and wider ascospores and longer asci. On the other hand, L. vestita is well-distinguished from L. investita by shorter, regularly 1-septate ascospores and shorter asci. Lentomitella investita resembles L. conoidea in the size of ascospores, but the latter differs by longer asci and mostly 1–2-septate ascospores with the first-formed septum in the middle or slightly above or below the middle. Lentomitella investita, L. vestita and L. cirrhosa were formerly treated as conspecific (von Arx 1952, R eblov a 2006). Based on molecular evidence the two latter are accepted as separate species in our study. Although no DNA sequence data of L. investita are available, given the size of asci and ascospores, the species is intermediate between L. vestita and L. cirrhosa and therefore regarded as distinct. Lentomitella cirrhosa differs from L. investita by longer and wider ascospores and longer asci. On the other hand, L. vestita is well-distinguished from L. investita by shorter, regularly 1-septate ascospores and shorter asci.

Notes: This collection from New Zealand is grouped in a monophyletic clade consisting of this and the three species L. magna, L. obscura and L. sulcata; all are characterised by 3-septate, ellipsoidal-fusiform, slightly inequilateral ascospores. It is most similar to L. sulcata, which also originates from New Zealand, in morphology of ascospores and conidia but differs in shorter ascospores and asci. For comparison of this clade at theRNA structural level see Discussion. The specimen M.R. 2953 was scarce and contained only a few ascomata; because no material is left after isolation and examination, and the culture is no longer available, this specimen is labelled Lentomitella sp. for the time being and its morphological description, illustration and DNA sequences are published in this study.
Specimen examined: New Zealand, South Island, Southland Distr., Catlins Coastal Rain Forest park, Haldane, Kiwi Walk along the Waipohatu stream, at the end of Waipohatu Road, 11 km W of Waikawa, 14 Mar. 2005, on decaying wood, M.R. 2953/NZ 500.

Our specimens from New Zealand and the Czech Republic fit well the description and illustration of T. fibrosa from Hong Kong, China, provided by Hyde et al. (2000), except that the ascospores do not possess a fibrillar sheath. The occurrence on water-saturated decayed wood (ICMP 15147, collected as dried drift-wood on the shores of a river) is consistent with the ecology of other freshwater species classified in this genus. The material from the Czech Republic was collected in the glacial cirque in the Sumava Mts., a unique locality with specific microclimatic conditions. The ascomata in our collections were larger than ascomata in the type collection (135–255 μm diam fide Hyde et al. 2000). The material collected in New Zealand was isolated in axenic culture, but only sterile dematiaceous mycelium was produced. Considering that cryptic speciation appears to be common in this lineage, the morphological differences in the ascospore wall, ecological differences, and the absence of molecular data from collections originating from the area of original description of T. fibrosa, the present collections are tentatively identified as this species, pending further investigations of accessions from the area of its description.