Download Copy a link to this page Cite this record

Samuels, G.J. 1978: Some species of Nectria having Cylindrocarpon imperfect states. New Zealand Journal of Botany 16(1): 73-82.

Reference record
Names_Fungi record source
Is NZ relevant
This record has descriptions
Show more

Click to collapse Details Info

Samuels, G.J. 1978: Some species of Nectria having Cylindrocarpon imperfect states. New Zealand Journal of Botany 16(1): 73-82.
Article

Click to collapse Descriptions Info

Additional specimens examined: NEW ZEALAND: Auckland, Franklin County, Hunua Ranges, Mangatangi Hill Rd, on decorticated wood, Dingley et al., 17 Apr. 1973 (PDD 34935, Samuels 73-30), Waitemata County, Waitakere Ranges, data as holotype (PDD 34936, Samuels 74-135).
Mycelium a ring of white hyphae around each perithecial base or not visible. Perithecia solitary or in groups of a few; white; globose to pyriform, 150-250µm in diam.; with an apical, sometimes slightly stellate, disc; collapsing by lateral pinching or not collapsing when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 15-25 µm wide. Surface view: textura intricata, walls of cells 1-2 µm thick. Longitudinal section: outer region c. 10 µwide, cells elliptic, 3-5 x 2-3 µm, walls of cells 1-2 µm thick; inner region 5-10 µm wide, cells tangentially compressed, 5-7 x 2-3 µm, walls 0.5-1 µm thick. Ostiolar disc 150-200 µm in diam., composed of agglutinated hyphae arising from the outer region of the perithecial wall; hyphae branched, septate with walls 1-2µm thick; groups of hyphae sometimes growing outwardly from the disc to form short, triangular hairs. Ostiolar canal piercing the ostiolar disc, periphysate. Asci clavate, (50-) 60-80 (-87) x (6-) 8-10 (-11) µm, 8-spored; apices rounded, simple, or with a poorly visible ring; bases rounded or pedicellate; ascospores forming throughout the length. Ascospores clavate, tapering from the rounded apical end to the pointed basal end or fusiform, (25-) 27-32 (-37) x 3.5-4 µm, 7-8-septate, smooth, not constricted at the septa, hyaline.
Characteristics in culture: Colony characteristics: CMD, 1-2cm; ME and PDA, 1-1.5 cm. Aerial mycelium lacking or sparse, white. Surface of colony on PDA shining and wrinkled. Colonies non-pigmented. Conidia forming within 2 weeks. Conidiophores arising from the surface of the colony, bearing a single phialide, unbranched (1-) 2-3-septate, straight, smooth, 70-135 (-170) µm long, (1.5-) 2-3 µm wide basally, subtending hypha 1.5-3 µm wide. Phialides (35-) 50-70 (-135) µm long, 1.5-2 µm wide at the unflared tip. Conidia cylindrical with rounded to pointed ends, 0-7-septate with 3-septate conidia predominating, (15-) 19-25 (-28) x 2-3µm, without a basal abscission scar; held in a hyaline drop of liquid.
Habitat: On decaying leaves and decorticated wood.
Perithecia 150-250 µm diametro, globosa vel pyriformia, alba, appendiculis brevibus, pluricellularibus, planatis, triangularibus et hyalinis vestita; asci cylindrici vel fusiformes, 60-80 x 8-10 µm; ascosporae 7-8-septatae, clavatae, 27-32 x 3.5-4µm, hyalinae. HOLOTYPUS: PDD 34934. Conidial state: Cylindrocarpon sp.

Etymology of the specific epithet: Refers to the clavate ascospores.

Notes: Whereas the preceding species discussed here are all members of the N. arenula-group, N. corynospora belongs to the N. subfalcata-group. Within that large group it is most closely related to N. peristomialis (Berkeley) Samuels and N. dentifera Samuels, both of which have an apical crown of hairs composed of agglutinated hyphae (Samuels 1976b). The ascospores of N. peristomialis are 1-4-septate, 25-32 x 4-6 µm, whereas those of N. dentifera are 1-septate and measure 6-8 x 3-4 µm. Nectria corynospora and N. parilis H. Sydow (ISOTYPE: Fungi exotici exsiccati 838, NY!) are very close to each other. Perithecia of N. parilis are white and are found on dicotyledonous leaves. The bicellular ascospores measure 15-21 x 2-3 µm and are finely spinulose.

Holotype: NEW ZEALAND: Auckland, Waitemata County, Waitakere Ranges, vic. Piha, Marowhara Loop Track, on dead leaf of Phormium tenax, Dingley et al., 17 Dec. 1974 (PDD 34934, Samuels 74-140).
Additional specimens examined: Data as holotype, Marguerite Track, on leaf of Gahnia sp., Dingley & Haydon, 21 Aug. 1974 (PDD 34055, Samuels 74-109); Little Barrier Island, track to summit of Mt Hauturu, on dead leaves of Astelia sp., Samuels 76-8, 22 Feb. 1976 (PDD 34940).
Mycelium not apparent. Perithecia solitary or in groups of a few, seated directly on the substrate; orange to brown; glabrous; globose, 340-400 µm diam., collabent or not collapsing when dry; yellow in 3% potassium hydroxide, becoming orange in lactophenol, reaction reversible.Perithecial wall 60-100 µm wide. Surface view: textura angularis, cells 10-15 µm in greatest dimension, walls c. 1 µm thick. Longitudinal section: outer region 50-90 µm wide, textura angularis, cells 7-15 µm in greatest dimension, walls c. 1 µm thick; inner region 10-15µm thick, cells fusiform. Perithecial papilla lacking, ostiolar canal periphysate. Asci clavate to fusiform, 100-120 x (7-) 14-17µm, 8-spored; apices rounded, simple; bases pointed, ascospores biseriate, forming throughout the length. Ascospores fusiform, 50-70 (-80) x 6-7 (-8) µm, (4-) 15-20-septate when discharged from asci, a few longitudinal septa may form, striate; not constricted at the septa; hyaline.
Characteristics in culture: Colony characteristics: Colony diameter: CMD, 2-3 cm; ME, just beginning to grow away from the inoculum; PDA 1-1.5 cm. Aerial mycelium lacking or sparse on CMD; acute, rope-like strands forming on ME and PDA. Colonies white on CMD, white to tan on ME and PDA. Ferrugineous pigment spreading into all three media. Non-conidial or conidia forming within 2-3 months on CMD and PDA; none on ME. Conidiophores arising from the aerial mycelium, bearing a single phialide, unbranched, 1-2-septate, 40-50 µm long, 3-4 µm wide basally, subtending hypha c. 2 µm wide. Phialides 35-40 (-45) µm long, 1.5-2 µm wide at the unflared tip. Conida cylindrical, (1-) 3-septate, 1-septate: 21-28 x 3-4µm, 3-septate: 21-35 x 3-4 µem, hyaline with or without a flattened, protuberant, basal, abscission scar, held in a drop of hyaline liquid.
Habitat: On decaying leaves of monocotyledons.
Perithecia 340-400 µm diametro, globosa, aurantiaca vel brunnea; asci clavati vel fusiformes, 100-120 x 14-17 µm, apice simplici; ascosporae 15-20 septatae, fusiformes, hyalinae striatae. HOLOTYPUS: PDD 31786. Conidial state: Cylindrocarpon sp.
Etymology of the specific epithet: Refers to the phragmosporous ascospores.
Holotype: NEW ZEALAND: Auckland, Waitemata County, Waitakere Ranges, Lucy Cranwell Track, vic. Kitakiki Stream, on dead leaf of Astelia sp., Dingley et al., 30 May 1973 (PDD 31786, Samuels 73-98).
Additional specimens examined: Location as holotype, 19 Jul. 1973, Samuels 73-141 (PDD 32423), Samuels 73-144 (PDD 31869), 9 Mar. 1976, Samuels 76-59 & McKenzie (PDD 34938) Samuels (76-137) & MacKay, 20 Aug. 1976 (PDD 36012), on flowering stalk of Phormium tenax, Samuels 76-138 & MacKay, 20 Aug. 1976 (PDD 36011).
Mycelium a ring of white to tan hyphae surrounding each perithecial base, with spreading, white conidiogenous hyphae. Perithecia scattered, solitary, or in groups of a few, seated directly on the substrate; orange to brown; smooth to slightly roughened with scales; globose to tympaniform (340-) 370-470 µm diam., collabent or not collapsing when dry; not changing colour in 3% potassium hydroxide. Perithecial wall (30-) 40-70 µm wide. Surface view: textura angularis, cells 7-15µm in greatest dimension, walls 0.5-1.0µm thick. Longitudinal section: outer region (20-) 50-60µm wide; textura angularis, cells may be rounded, 10-15 µm in greatest dimension, walls 0.5-1µm thick, pigmented; inner region 15-20 µm wide, cells flattened, 10-15 x 3-4 µm, walls 0.5-1 µm thick, non-pigmented. Perithecial papilla lacking, ostiolar canal periphysate. Asci clavate, 80-100 x 9-18 µm, 6-8-spored; apices rounded, simple; bases rounded to pointed; ascospores biseriate, or biseriate above and uniseriate below, forming throughout the length. Ascospores fusiform, (25-) 28-35 (-40) x (4-) 5-6 (-6.5) µm, (3-) 6-8 (-10)-septate when discharged from asci, striate, constricted at the median septum, hyaline.
Characteristics in culture: Colony characteristics: Colony diameter: CMD, 3-4 cm; ME, 1-1.5 cm; PDA, 1.5-2 cm. Aerial mycelium lacking or sparse on CMD, erect, rope-like strands forming on ME and PDA. Colonies white on CMD, pale pink to tan on ME, and tan to brown on PDA. Orange-brown drops of liquid scattered in aerial mycelium on ME and PDA. Surface of colony on PDA wrinkled and crustose. Ferrugineous pigment spreading into all three media. Conidia forming poorly after 1 month on CMD, after 4-6 weeks on PDA; none on ME. Conidiophores arising from the aerial hyphae and from the surface of the colony, bearing a single phialide, unbranched, aseptate, (17-) 21-33 (-35) µm long, 2-3µm wide basally, 1.5µm wide at the unflared opening of the phialide; subtending hypha 2-3 µm wide. Conidia cylindrical, (0-) 1-7-septate, 3-septate predominating, 0-septate: 7-11 x 2-3 µm, 1-septate: 9-15 x 2-3 (-4) µm, 2-septate: 17-20 (-26) x 3-4 µm, 3-septate: 19-28 (-35) x 3-4µm, 4-6-septate: 22-34 (-37) x 3-4 µm, 7-septate: 35-40 x 3-4 µm, hyaline, with a protuberant, flattened, basal, abscission sear; held in a hyaline drop of liquid.
Habitat: On decaying leaves of Phormium tenax.
Perithecia 370-470µm diametro, globosa vel tympaniformia, aurantiaca; asci clavati vel fusiformes, 80-100 x 9-18µm, apice simplici; ascosporae 6-8 septatae, fusiformes, rectae vel curvatae, 28-35 x 5-6µm, hyalinae, striatae. HOLOTYPUS: PDD 34937. Conidial state: Cylindrocarpon sp.

Etymology of the specific epithet: Refers to the multiseptate ascospores.

Notes: The description of Calonectria callorioides Penzig et Saccardo is suggestive of N. multiseptata. The type specimen of C. callorioides (PAD!) comprises a few red thalli of what appears to be a red alga (? Porphyridiales) and erect, black hairs of aChaetosphaeria. I did not find the asci or ascospores that were described and illustrated by Penzig and Saccardo.
Nectria multiseptata and Calonectria ochraceo-pallida (Berkeley et Broome) Saccardo are superficially similar. I have examined two New Zealand collections of C. ochraceo-pallida (PDD 3279, PDD 34941) and have found the cells of the wall to be very small and indistinct; this observation agrees well with the description of the species by Weese (1914c). One of the New Zealand collections (PDD 34941) produced an Acremonium sp. in cultures derived from single ascospores.

Holotype: NEW ZEALAND: Auckland, Waitakere Ranges, vic. Piha, Marowhara Loop Track, on leaves of Phormium tenax, Dingley, Samuels (74-136) & Francis, 17 Dec. 1974 (PDD 34937).

Click to collapse Metadata Info

1cb0e322-36b9-11d5-9548-00d0592d548c
reference
Names_Fungi
26 September 2003
Click to go back to the top of the page
Top