Samuels, G.J. 1978: Some species of Nectria having Cylindrocarpon imperfect states. New Zealand Journal of Botany 16(1): 73-82.
Details
Associations
Descriptions
Characteristics in culture: Colony characteristics: CMD, 1-2cm; ME and PDA, 1-1.5 cm. Aerial mycelium lacking or sparse, white. Surface of colony on PDA shining and wrinkled. Colonies non-pigmented. Conidia forming within 2 weeks. Conidiophores arising from the surface of the colony, bearing a single phialide, unbranched (1-) 2-3-septate, straight, smooth, 70-135 (-170) µm long, (1.5-) 2-3 µm wide basally, subtending hypha 1.5-3 µm wide. Phialides (35-) 50-70 (-135) µm long, 1.5-2 µm wide at the unflared tip. Conidia cylindrical with rounded to pointed ends, 0-7-septate with 3-septate conidia predominating, (15-) 19-25 (-28) x 2-3µm, without a basal abscission scar; held in a hyaline drop of liquid.
Etymology of the specific epithet: Refers to the clavate ascospores.
Notes: Whereas the preceding species discussed here are all members of the N. arenula-group, N. corynospora belongs to the N. subfalcata-group. Within that large group it is most closely related to N. peristomialis (Berkeley) Samuels and N. dentifera Samuels, both of which have an apical crown of hairs composed of agglutinated hyphae (Samuels 1976b). The ascospores of N. peristomialis are 1-4-septate, 25-32 x 4-6 µm, whereas those of N. dentifera are 1-septate and measure 6-8 x 3-4 µm. Nectria corynospora and N. parilis H. Sydow (ISOTYPE: Fungi exotici exsiccati 838, NY!) are very close to each other. Perithecia of N. parilis are white and are found on dicotyledonous leaves. The bicellular ascospores measure 15-21 x 2-3 µm and are finely spinulose.
Characteristics in culture: Colony characteristics: Colony diameter: CMD, 2-3 cm; ME, just beginning to grow away from the inoculum; PDA 1-1.5 cm. Aerial mycelium lacking or sparse on CMD; acute, rope-like strands forming on ME and PDA. Colonies white on CMD, white to tan on ME and PDA. Ferrugineous pigment spreading into all three media. Non-conidial or conidia forming within 2-3 months on CMD and PDA; none on ME. Conidiophores arising from the aerial mycelium, bearing a single phialide, unbranched, 1-2-septate, 40-50 µm long, 3-4 µm wide basally, subtending hypha c. 2 µm wide. Phialides 35-40 (-45) µm long, 1.5-2 µm wide at the unflared tip. Conida cylindrical, (1-) 3-septate, 1-septate: 21-28 x 3-4µm, 3-septate: 21-35 x 3-4 µem, hyaline with or without a flattened, protuberant, basal, abscission scar, held in a drop of hyaline liquid.
Characteristics in culture: Colony characteristics: Colony diameter: CMD, 3-4 cm; ME, 1-1.5 cm; PDA, 1.5-2 cm. Aerial mycelium lacking or sparse on CMD, erect, rope-like strands forming on ME and PDA. Colonies white on CMD, pale pink to tan on ME, and tan to brown on PDA. Orange-brown drops of liquid scattered in aerial mycelium on ME and PDA. Surface of colony on PDA wrinkled and crustose. Ferrugineous pigment spreading into all three media. Conidia forming poorly after 1 month on CMD, after 4-6 weeks on PDA; none on ME. Conidiophores arising from the aerial hyphae and from the surface of the colony, bearing a single phialide, unbranched, aseptate, (17-) 21-33 (-35) µm long, 2-3µm wide basally, 1.5µm wide at the unflared opening of the phialide; subtending hypha 2-3 µm wide. Conidia cylindrical, (0-) 1-7-septate, 3-septate predominating, 0-septate: 7-11 x 2-3 µm, 1-septate: 9-15 x 2-3 (-4) µm, 2-septate: 17-20 (-26) x 3-4 µm, 3-septate: 19-28 (-35) x 3-4µm, 4-6-septate: 22-34 (-37) x 3-4 µm, 7-septate: 35-40 x 3-4 µm, hyaline, with a protuberant, flattened, basal, abscission sear; held in a hyaline drop of liquid.
Etymology of the specific epithet: Refers to the multiseptate ascospores.
Notes: The description of Calonectria callorioides Penzig et Saccardo is suggestive of N. multiseptata. The type specimen of C. callorioides (PAD!) comprises a few red thalli of what appears to be a red alga (? Porphyridiales) and erect, black hairs of aChaetosphaeria. I did not find the asci or ascospores that were described and illustrated by Penzig and Saccardo.
Nectria multiseptata and Calonectria ochraceo-pallida (Berkeley et Broome) Saccardo are superficially similar. I have examined two New Zealand collections of C. ochraceo-pallida (PDD 3279, PDD 34941) and have found the cells of the wall to be very small and indistinct; this observation agrees well with the description of the species by Weese (1914c). One of the New Zealand collections (PDD 34941) produced an Acremonium sp. in cultures derived from single ascospores.