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Chun, Byung Hee; Lee, Yunhee; Jin, Hyun Mi; Jeon, Che Ok 2017: Cloacibacterium caeni sp. nov., isolated from activated sludge. International Journal of Systematic and Evolutionary Microbiology 67(6): 1688-1692.

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Chun, Byung Hee; Lee, Yunhee; Jin, Hyun Mi; Jeon, Che Ok 2017: Cloacibacterium caeni sp. nov., isolated from activated sludge. International Journal of Systematic and Evolutionary Microbiology 67(6): 1688-1692.
10.1099/ijsem.0.001841
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Cells are Gram-stain-negative, facultatively anaerobic, non-motile rods (0.4–0.6 µm in width and 1.1–1.4 µm in length). Catalase- and oxidase-positive. On R2A agar after 2 days of incubation at 37 °C, colonies are round, convex and yellow, with entire margins. Growth occurs at 20–40 °C (optimum, 37 °C) and pH 6.6–8.2 (optimum, pH 7.0) and in R2A broth supplemented with 0–1 % (w/v) NaCl (optimum, 0 %). Yellow carotenoid-type pigments are produced but flexirubin-type pigments are not. Hydrolyzes aesculin, but not gelatin, Tween 40 and Tween 80. Alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, α-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-glucosidase and β-glucosidase activities are positive, but lipase (C14), α-galactosidase, β-galactosidase, β-glucuronidase, α-mannosidase, α-fucosidase, arginine dihydrolase, urease and N-acetyl-β-glucosamindase activities are negative. Indole production and assimilation of d-glucose, d-mannose and d-maltose are positive, but reduction of nitrate to nitrite and assimilation of l-arabinose, d-mannitol, potassium gluconate, capric acid, N-acetyl-glucosamine, adipic acid, malate, trisodium citrate and phenylacetic acid are negative. The major cellular fatty acid is iso-C15 : 0. MK-6 is the sole isoprenoid quinone.
The type strain is B6T (=KACC 18988T=JCM 31714T), isolated from activated sludge of a wastewater treatment plant in South Korea. The DNA G+C content of the type strain is 31.5 mol%.

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8 April 2025
8 April 2025
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