Notes: Fusarium agreste, previously recognized as lineage Fusarium sp. nov. 8 in Laraba et al. (2021), resolved as the closest phylogenetic sibling of F. sambucinum, clustering in an intermediate position between F. sambucinum and F. symmetricum, the three species producing relatively fast growing, red colonies on PDA and up to 5-septate sporodochial conidia of similar width. Nevertheless, phylogenetic analyses, coupled with multilocus and most singlelocus coalescent-based analyses support the recognition of this clade as a genetically exclusive novel taxon, which is reinforced by morphological features. Fusarium agreste can be distinguished from F. sambucinum and F. symmetricum by its longer and robust conidia with well-developed foot-cells and by the production of 0–1-septate microconidia on aerial conidiophores. Both F. agreste and F. symmetricum are so far known to occur in the Southern Hemisphere; F. agreste in South Africa and New Zealand, while the latter species is known from Cactaceae in South America. In contrast, F. sambucinum has a worldwide distribution and has been recorded from more than 150 different substrates, including soil, animals (among them insects, and mammals, including human), and more than 130 plant host; mostly from Fabaceae, Poaceae, Pinaceae and Solanaceae (Farr et al. 2023).