Cytospora pruinosa is distributed worldwide on Fraxinus, Syringa and Olea (Adams et al. 2006, Fan et al. 2020). In the ITS, act1, rpb2, tef1-α and tub2 gene trees, the BI and ML phylogenies all placed it in a distinct clade, with high support (ML/ BI = 99/1, 100/1, 100/1, 100/1 and 100/1, respectively). No type material could be located for this species, but a lectotype can be chosen from Fries, namely Scleromycetes Sueciae 82, which is mentioned in the sanctioning publication (Fries 1823). In the present study, CBS 200.42 is treated as a reference isolate by the same host species and collected continent as type. Valsa cypri which commonly considered a synonym of Cytospora pruinosa is described from Ligustrum vulgare (Défago 1942, Hayova & Minter 1998b), but this needs to be confirmed with sequence data for isolates from the type host.