Lasionectria vulpina (Cooke) Rossman & Samuels 1999
Details
Nomenclature
Classification
Associations
Descriptions
Mycelium white, scanty, spreading over the substrate. Perithecia solitary or in groups of a few, orange, glabrous or hirsute, urniform, (155-) 190-220 (-230) µm high x (200-) 247-270 (-375) µm wide, becoming collabent when dry, not changing colour in 3% potassium hydroxide. Perithecial wall 15-25 (-40) µm thick. Surface view: cells prosenchymatous, walls 1-2 µm thick. Longitudinal section: cells elliptical, 5-7 µm long x 2-4 µm wide, walls 1-2 µm thick, cells becoming progressively more flattened and thin-walled toward the interior. Perithecial hairs lacking or composed of from one to several fasciculate hyphae, 10-70 wm long, 20-40 µm wide basally, orange to brown; hyphae unbranched, septate, 3-5 µm wide with walls c. 1 µm thick, arising from the surface of the perithecial wall. Perithecial papilla lacking or very short, composed of narrow hyphal elements arising from the inner region of the perithecial wall; ostiolar canal periphysate. Asci broadly cylindrical to clavate, 37-58 x 6-8 (-10) µm, 8-spored; apices flat, with a ring; bases rounded; ascospores obliquely uniseriate, becoming irregularly arranged, forming throughout the length. Ascospores fusiform-ellipsoidal, (7-) 8-11 (-13) x 3-4 µm, equally 2-celled, 2 drops in each cell, striate, non-constricted or slightly constricted at the septum, hyaline.
CHARACTERISTICS IN CULTURE CMD: Ascospores germinating overnight, one, short germ tube arising from one end of each ascospore. Colonies 1.5-2.0 cm diam., translucent to opaque, white to pale pink, aerial mycelium loose, abundant pink to salmon slime on the surface of the colony, margin entire to slightly undulate, aromatic, medium non-pigmented. ME: 1 cm or less diam., opaque, pink to salmon, aerial mycelium loose or forming poorly developed, hyphal, rope-like strands, abundant pink to salmon slime on the surface of the colony, margin undulate, aromatic, medium non-pigmented. OA: 1.5-2.5 cm diam., opaque; yellow to salmon in the centre, white elsewhere; aerial mycelium as erect, outwardly directed, hyphal, rope-like strands, margin undulate, aromatic, medium non-pigmented. WAS: 2 cm diam., translucent, aerial mycelium sparse, white, margin entire, non-aromatic, medium non-pigmented. Phialophores arising on CMD, ME, OA, and WAS from the surface of the agar and from the aerial mycelium; monophialidic, unbranched, aseptate, straight, smooth, 49-57 µm long, 2.5-3 µm wide basally, subtending hypha 1.5-2 µm wide. A solitary phialide constituting the terminal 44-52 µm of each phialophore, 1-1.5 µm wide at the unflared tip. Conidial production abundant. Phialoconidia ellipsoidal to cylindrical, 5-7 x 1.5-2 µm, unicellular, hyaline, lacking a basal abscission scar. Arising in basipetal succession, held in a solitary pink to salmon (on CMD, ME, and OA) or hyaline (on WAS) drop of liquid.
Nectria vulpina has not previously been grown in pure culture. The above description of the characteristics in culture is based on colonies derived from three ascospores of one collection.