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Chuang, Shu-Cheng; Dobhal, Shefali; Alvarez, Anne M.; Arif, Mohammad: Three new species, Xanthomonas hawaiiensis sp. nov., Stenotrophomonas aracearum sp. nov., and Stenotrophomonas oahuensis sp. nov., isolated from the Araceae family. Frontiers in Microbiology 15

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Chuang, Shu-Cheng; Dobhal, Shefali; Alvarez, Anne M.; Arif, Mohammad: Three new species, Xanthomonas hawaiiensis sp. nov., Stenotrophomonas aracearum sp. nov., and Stenotrophomonas oahuensis sp. nov., isolated from the Araceae family. Frontiers in Microbiology 15
10.3389/fmicb.2024.1356025
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Colonies of S. aracearum strain A5588T are dark yellow (Mustard, Hex code #E8B828), irregular shape, butyrous consistency, smooth surface, raised relief with entire margins, and 0.4–0.5 mm (average 0.45) in diameter on YDC medium plates after incubation at 28°C for 2 days. Cells are gram-negative and able to utilize D-maltose, D-cellobiose, gentiobiose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, α-D-glucose, D-mannose, 1% sodium lactate, D-serine, troleandomycin, rifamycin SV, gelatin, lincomycin, guanidine HCl, vancomycin, tetrazolium violet, tetrazolium Blue, α-ketoglutaric acid, L-malic acid, bromo-succinic acid, acetic acid, and aztreonam. Cells grow under pH 6 and 1% NaCl but neither at pH 5 nor 8% NaCl. In the contrary, cells are unable to oxidize sucrose, D-turanose, stachyose, D-raffinose, α-D-lactose, D-melibiose, Β-methyl-D-glucoside, N-acetyl-β-D-mannosamine, N-acetyl-neuraminic acid, D-galactose, 3-methyl-glucose, inosine, fusidic acid, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, glycerol, D-glucose-6-PO4, D-aspartic acid, D-serine, minocycline, L-arginine, L-aspartic acid, L- glutamic acid, L-histidine, L-pyroglutamic acid, L-serine, pectin, D-galacturonic acid, D-gluconic acid, mucic acid, quinic acid, D-saccharic acid, p-hydroxy-phenylacetic acid, D-lactic acid methyl ester, L-lactic acid, citric acid, D-malic acid, nalidixic acid, potassium tellurite, γ-aminobutryric acid, α-hydroxybutyric acid, α-ketobutyric acid, β-hydroxy-D, L-butyric acid, acetoacetic acid, formic acid, sodium butyrate, and sodium bromate. Some utilization of carbon sources and chemical components, such as dextrin and glucuronamide showed faded positive results after growing A5588T cell suspension in GEN III Microplate (Biolog Inc., Hayward, CA, USA) at 28°C for 24 h. S. aracearum sp. nov. was sensitive to six tested antibiotics, namely, bacitracin (50 mg/mL), chloramphenicol (50 mg/mL), gentamicin (50 mg/mL), kanamycin (50 mg/mL), tetracycline (40 mg/mL), and polymyxin B sulfate (50 mg/mL) but resistant to penicillin (50 mg/mL) on NA plates. The genome size of type strain A5588T is 4.33 Mbp with 66.44 mol% of DNA G + C content.
The type strain A5588T = D-61-1LT = ICMP 25025T = LMG 33202T was isolated from Anthurium (Araceae family) in 1985 in Hawaii, USA.
Colonies of the S. oahuensis strain A5586T are dark yellow (Butterscotch, Hex code #FABD02), circular shape, butyrous consistency, smooth surface, flat relief with undulate margins, and 0.4–0.7 mm (average 0.55) in diameter on YDC medium plates after incubation at 28°C for 2 days. Cells are gram-negative and able to utilize dextrin, D-maltose, D-trehalose, D-cellobiose, gentiobiose, Β-methyl-D-glucoside, D-salicin, N-acetyl-D-glucosamine, α-D-glucose, D-mannose, 1% sodium lactate, gelatin, glycyl-L-proline, lincomycin, guanidine HCl, vancomycin, tetrazolium violet, tetrazolium blue, citric acid, α-ketoglutaric acid, L-malic acid, bromo-succinic acid, lithium chloride, propionic acid, acetic acid, and aztreonam. Cells grow under the conditions of pH 6, 1% NaCl, or 4% NaCl but cells survive neither pH 5 nor 8% NaCl solution. In contrast, cells are unable to oxidize sucrose, stachyose, D-raffinose, N-acetyl-β-D-mannosamine, N-acetyl-neuraminic acid, D-galactose, 3-methyl-glucose, inosine, D-fucose, L-fucose, L-rhamnose, inosine, fusidic acid, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, glycerol, D-glucose-6-PO4, D-aspartic acid, D-serine, rifamycin SV, minocycline, L-arginine, L-aspartic acid, L- glutamic acid, L-histidine, L-pyroglutamic acid, L-serine, pectin, D-galacturonic acid, D-gluconic acid, D-glucuronic acid, mucic acid, quinic acid, D-saccharic acid, p-hydroxy-phenylacetic acid, D-lactic acid methyl ester, L-lactic acid, D-malic acid, nalidixic acid, potassium tellurite, γ-amino-butryric acid, α-hydroxy-butyric acid, β-hydroxy-D, L-butyric acid, α-keto-butyric acid, acetoacetic acid, formic acid, and sodium bromate. Some utilization of carbon resources and chemical components, such as D-turanose and sodium butyrate, showed faded positive results after growing A5586T cell suspension in GEN III Microplate (Biolog Inc., Hayward, CA, USA) at 28°C for 24 h. S. oahuensis sp. nov. was sensitive to seven tested antibiotics, namely, bacitracin (50 mg/mL), chloramphenicol (50 mg/mL), gentamicin (50 mg/mL), kanamycin (50 mg/mL), penicillin (50 mg/mL), tetracycline (40 mg/mL), and polymyxin B sulfate (50 mg/mL). The genome size of type strain A5586T is 4.68 Mbp, which includes a chromosome (4.62 Mbp) and a plasmid (60.78 Kbp). The DNA G + C content of the type strain is 65.3 mol%
The type strain A5586T = D-31T = ICMP 25024T = LMG 33201T was isolated from Anthurium (Araceae family) in 1981 in Hawaii, USA.
Colonies of the type strain A6251T are yellow (Honey, Hex code #FFC30B), circular shape, mucoid consistency, smooth surface, convex relief with entire margins, and 0.3–0.6 (avg. 0.45) mm in diameter on yeast dextrose calcium carbonate (YDC) medium plate after incubating at 28°C for 2 days. Cells are gram-negative and able to utilize dextrin, D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-turanose, α-D-lactose, D-melibiose, Β-methyl-D-glucoside, D-salicin, N-acetyl-D-glucosamine, α-D-glucose, D-mannose, D-fructose, D-galactose, L-fucose, 1% NaCl, 1% sodium lactate, glycerol, gelatin, L-glutamic acid, lincomycin, pectin, quinic acid, vancomycin, tetrazolium violet, tetrazolium blue, citric acid, bromo-succinic acid, lithium chloride, Tween 40, and acetic Acid. In contrast, cells are unable to oxidize stachyose, D-raffinose, N-acetyl-β-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl-neuraminic acid, 8% NaCl, inosine, fusidic acid, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, D-aspartic acid, minocycline, L-arginine, L-histidine, L-pyroglutamic acid, guanidine HCl, D-gluconic acid, mucic acid, D-saccharic acid, p-hydroxy-phenylacetic acid, D-lactic acid methyl ester, α-keto-glutaric acid, D-malic acid, γ-aminobutryric acid, α-hydroxybutyric acid, α-ketobutyric acid, formic acid, sodium butyrate, and sodium bromate. Some utilization of carbon resources and chemical components showed borderline results or inconsistency between two strains after growing cell suspension in GEN III Microplate (Biolog Inc., Hayward, CA, USA) at 28°C for 24 h. X. hawaiiensis sp. nov. is sensitive to seven tested antibiotics, including bacitracin (50 mg/mL), chloramphenicol (50 mg/mL), gentamicin (50 mg/mL), kanamycin (50 mg/mL), penicillin (50 mg/mL), tetracycline (40 mg/mL), and polymyxin B sulfate (50 mg/mL). The genome size of type strain A6251T is 4.88 Mbp with 68.93 mol% of DNA G + C content.
The type strain A6251T = D-93T = ICMP 25022T = LMG 33200T was isolated from Spathiphyllum (Araceae family) in 1985 in Hawaii, USA. Another strain A2111 = D-194 = ICMP 25023 = LMG 33199 was isolated from Colocasia (Araceae family) in 1986 in Hawaii, USA.

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27 October 2024
14 June 2025
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