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Samuels, G.J.; Rossman, A.Y. 1987: Studies in the Amphisphaeriaceae (sensu lato) 2. Leiosphaerella cocoes and two new species of Oxydothis on palms. Mycotaxon 28(2): 461-471.

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Samuels, G.J.; Rossman, A.Y. 1987: Studies in the Amphisphaeriaceae (sensu lato) 2. Leiosphaerella cocoes and two new species of Oxydothis on palms. Mycotaxon 28(2): 461-471.
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ADDITIONAL SPECIMENS EXAMINED (all on Rhopalostylis sapida). NEW ZEALAND: Northland, Hokianga County, Omahuta State Forest, Samuels, 8 May 1983 (PDD 45798), second collection, Samuels, Matsushima & Petersen, 9 May 1983 (PDD 45799); Hokianga County, Waipoua State Forest, 30 May 1982 (PDD 44394), second collection, Samuels, Matsushima & Petersen, 12 May 1983 (PDD 45786, NY), third collection, Samuels 13 May 1983 (PDD 45777). Coromandel, Thames, Kauaeranga Valley, Samuels & Petersen, 1 May 1983 (PDD 46262, 46263).
Perithecia immersed in host tissue below slightly raised and blister-like, non-blackened lesions with only minute black dots indicating the perithecial openings, solitary or gregarious; each surrounded by stromatic tissue that lacks definite orientation; forming within the hypodermic, 3-4 cell layers below the epidermis and with 1 cell layer of hypodermal cells below each perithecium or seated directly on the vascular tissue; with brown hyphal cells growing into underlying vascular tissues. Cells of stroma pseudoparenchymatous, 812 µm in greatest dimension, walls < 1 µm thick, brown. Perithecial body 220-280 µm long x ca. 125 µm high, with long axis parallel to the surface of the host and perpendicular to the ostiolar canal; ostiolar canal 110-175 µm long. Perithecial wall not easily distinguished from the surrounding stromatic tissue, consisting of a ca. 10 µm wide region of elliptical cells 12 x 4.5 µm, with walls < 1 µm thick and dark brown; perithecial papilla composed of 5 µm wide hyphal elements arranged in a palisade; ostiolar canal periphysate; periphyses arising from a tissue distinct from the paraphyses. Paraphyses persisting among mature asci or deliquescing, much branched and anastomosed, ca. 2 µm wide, cells 10-15 µm long. Asci (130-)165-235(-285) x (7-)13.519.7(22) µm, cylindrical, apical ring J+ Melzer's, wedge-shaped, 2-3 µm wide x 2-2.5 µm long; 8-spored, ascospores biseriate to multiseriate, completely filling each ascus or up to 50 µm of the ascal base devoid of ascospores. Ascospores (65)74-88(-95) x (5.5-)6-7.2(-8) µm, narrowly fusiform with ends apiculate but not ciliate, with a single, median septum, hyaline, smooth.
Anamorph. None known.
KNOWN DISTRIBUTION. New Zealand (North Island: Northland, Coromandel).
HABITAT. On leaf midrib of Rhopalostylis sapida.
Oxydothis sabalensis (Cooke) Petrak similis sed ascosporae differt, (65-)74-88(-95) x (5.5-)6-7.2(-8) µm.
Holotypus. PDD 45779.
HOLOTYPE. NEW ZEALAND: Northland, Hokianga County, vic. Mangamuka Bridge, Omahuta State Forest, at forest headquarters, on Rhopalostylis sapida, Samuels (83-507), 11 May 1983 (PDD 45779, Isotype NY).
ADDITIONAL SPECIMENS EXAMINED (all on Rhopalostylis sapida). NEW ZEALAND: Northland, Waipoua State Forest, 4 collections [PDD 44394, 45777, 45783, 45786 (NY)]; Omahuta State Forest, Samuels, 8 May 1983 (PDD 45798). Auckland, Waitemata City, Waitakere Ranges, Cascades, Samuels, Horak & Johnston, 6 May 1981 (PDD 42057); Manukau City, vic. Clevedon, Morehu Reserve, Johnston, Samuels & Petersen, 5 May 1983 (PDD 44744).
Perithecia immersed in host tissue below slightly raised and blister-like, blackened lesions with only minute black dots indicating the perithecial openings, aggregated in groups of several in a common stroma within the hypodermis, 1 cell layer below the epidermis and with two layers of hypodermal cells below each perithecium; with brown hyphal cells growing into underlying vascular tissues. Stroma compact, 80-100 µm thick between perithecia, cells pseudoparenchymatous, 10-25 µm long x 4-5 µm wide, walls ca. 1.5 µm thick, brown, arranged in distinct files that are perpendicular to the surface of the host. Perithecial body 350-400 µm long x 150-170 µm high, with long axis parallel to the surface of the host and perpendicular to the ostiolar canal; ostiolar canal 35-80 µm long; adjacent perithecia discharging through a common ostiolar canal. Perithecial wall 5-6 µm wide, composed of a few layers of very narrow and flattened cells with light brown walls; perithecial papilla composed of 5 µm wide hyphal elements arranged in a palisade; ostiolar canal periphyste, periphyses 20-25 µm long, apparently arising from a tissue distinct from the paraphyses. Paraphyses persisting among mature asci or deliquescing, much branched and anastomosed, 1.5-4 µm wide, cells 10-15 µm long. Asci (125-)150-180(-200) x (6-) 6.5-7.5(-8) µm, cylindrical, apical ring J+ Melzer's, wedge-shaped, 2-2.5 µm wide x 1-1.5 µm long; 8-spored, ascospores biseriate to multiseriate, completely filling each ascus or up to 50 µm of the ascal base devoid of ascospores. Ascospores (49-)55.2-64.7(-77) x (2.5-)3.5-4(-4.5) µm, narrowly fusiform with ends apiculate but not ciliate, with a single, median septum, hyaline, smooth. Anamorph. Selenosporella sp.
CHARACTERISTICS IN CULTURE. Colonies grown one month at 20°C, diffuse daylight. CMD: 2-3.5 cm diam, scant aerial mycelium, with a few, hemispherical stromatic masses; center of colony dark olivaceous, margin colorless to pale olivaceous grey. OA: no aerial mycelium formed, black stromatic masses with developing perithecia forming throughout the colony. Conidia forming abundantly on CMD on stromatic masses or in aerial mycelium; none forming on OA. Conidiophores macronematous, mononematous, 30-45 x 4-6 µm wide at base, with (1-)2-3 septa, unbranched or once branched; brown olivaceous and thick-walled below; thin walled and nearly colorless above. Conidiogenous cells 10-15 µm long, finely denticulate, with a minute, refractive scar remaining after conidial dehiscence. Conidia (17-)23-27(-29) x 1-1.5(-2) µm, arcuate, without obviously differentiated apex or base, unicellular, colorless.
KNOWN DISTRIBUTION. New Zealand (North Island: Northland, Auckland).
HABITAT. On leaf midrib of Rhopalostylis sapida Wendl. & Drude.
Oxydothis sabalensis (Cooke) Petrak similis sed ascosporae differt, (49-)55.2-64.7(-77) x (2.5-)3.5-4 (-4.5) µm.
Holotypus. PDD 46266.
Status anamorphosis. Selenosporella sp.
NOTES. Oxydothis selenosporellae has much narrower and shorter ascospores and a smaller ring than 0. rhopalostylidis. Paraphyses of 0. selenosporellae tend to be broader and to have longer cells than the paraphyses of 0. rhopalostylidis. Both species have larger ascospores than 0. sabalensis (Fig. 4 D).
Perithecia of 0. selenosporellae consistently form only a single cell layer below the epidermis and in a compact stroma whereas perithecia of 0. rhopalostylidis form more deeply and lack a well developed stroma. The two species are intermingled on palm leaf midribs.
HOLOTYPE. NEW ZEALAND: Auckland, Waitakere Ranges, Waitemata City, Huia, Mill Bay, on Rhopalostylis sapida, Samuels (83-60), Matsushima & Petersen, 3 May 1983 (PDD 46266, Isotype NY).

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