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Samuels, G.J. 1985: Four new species of Nectria and their Chaetopsina anamorphs. Mycotaxon 22: 13-32.

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Samuels, G.J. 1985: Four new species of Nectria and their Chaetopsina anamorphs. Mycotaxon 22: 13-32.
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ADDITIONAL SPECIMEN EXAMINED. NEW ZEALAND: Gisborne, Urewera National Park, Lake Waikaremoana, track to Lake Waikare-iti, on decaying leaf of Astelia sp., G J. Samuels (83-161), P.R. Johnston, T. Matsushima & A.Y. Rossman, 29 May 1983 (PDD 44236).
Conidiophores mononematous, scattered, setiform, erect, unbranched, red-brown, becoming yellow in 100% lactic acid, 200-230 µm long, tapering from 8-10 µm wide just above the swollen, 10-15µm wide base to 4-5µm wide at tip; wall ca. 2 µm wide at base, thinning to <0.5 µm wide at tip; apex sterile; bearing few short lateral branches at or below the middle; branches formed of many small, <5 µm wide, thin-walled, non-pigmented cells, each cell producing or more phialides; phialides densely clustered, subglobose to ampulliform, (10-) 15-20(-22) µm long x 2.5-3.0 µm wide at base, monoblastic, tip 0.5-1.0 µm wide, lacking obvious periclinal thickening, not flared. Conidia oblong, (5.0-) 5.6-7.2(-8.5) x1.0-1.3 (-1.7) µm, lacking an obvious basal abscission scar, unicellular, hyaline, held in colorless slime. Sterile setae abundant, similar to conidiophores but lacking phialides and conidia. Perithecia scattered, solitary, superficial, non-stromatic, mycelium not evident; pyriform, apex acute, not collapsing when dry, smooth, red; remaining red in 3% KOH, becoming yellow in 100% lactic acid, (128-)139-190(-205) µm high x (115-)122-180(-185) µm wide. Cells at surface of perithecial wall textura epidermoidea, walls unevenly thickened, adjacent cells connected by pores or very thin regions of the wall. Perithecial wall ca. 10 µm wide, formed of single region of intertwined hyphae; cells in section elliptical to fusiform, lumens 5-8 µm long x 1-2 µm wide; wall ca. 2 µm thick, pigmented; cells of inner ca. 5 µm elongated to fusiform, wall <0.5 µm thick, non-pigmented. Perithecial apex comprising + elliptical cells at the surface; cells within arranged in a palisade of hyphae with rounded, 1-2 µm wide tips; hyphal elements merging with periphyses within. Perithecia anchored to substrate by hyphae arising from surface of lower perithecial wall. Asci clavate, (35-)38-43(-45) x 4.5-6.0(-8.0) µm, apex with a ring; 8- spored, ascospores 2-seriate, entirely filling each ascus or up to 10 µm of ascal base empty. Ascospores elliptical, (7.0-)7.8-9.3(-10.0) x 1.9-2.7(-3.0) µm, equally 2-celled, not constricted at the septum, smooth, hyaline.
CHARACTERISTICS IN CULTURE. CMD, PDA; OA: 1 cm diam, flat, waxy, opaque, lacking aerial mycelium, Sienna (Rayner 8) in center, margin white (on PDA, entire colony white). Conidiophores forming profusely on all media, macronematous, mononematous, setose, unbranched, smooth; red-brown, becoming yellow in 100% lactic acid, (107-)125-165(-175) µm long x 10-12 µm wide at base x 4-5 µm wide at subacute tip, (1-)6-9-septate; wall ca. 3 µm wide at base x <0.5 µm wide at apex. Phialides arising in a compact cluster in the upper third of the conidiophore less frequently terminating the conidiophore. Phialides arising from short, thin-walled, non-pigmented lateral branches of the conidiophore, with an enlarged, ca. 3 µm wide, subglobose base and an abruptly narrowed, 1 µm wide neck, smooth, thin-walled, non-pigmented, tip lacking periclinal thickening or periclinal thickening barely visible, not flared. Phialides. also arising singly or in + verticillately branched whorls of 2-3 from hyphae on surface of agar, subglobose to ampulliform, (10-) 15-20(-22) µm long; tip 1 µm, wide, with periclinal thickening; base 1.5-2.0 µm wide, smooth or with yellow (in lactic acid) granular incrustations along the lower 2/3 of the phialide and subtending hypha. Conidia oblong to cylindrical, (6.5-)9.3-17.3(-22.0) x 2.0-2.5(-3.0) µm, lacking an obvious basal abscission scar, unicellular, hyaline.
KNOWN DISTRIBUTION. Anamorph cosmopolitan, teleomorph known only from New Zealand.
HABITAT. Decaying leaves of Collospermum hastatum (Col.) Skottsb. and Astelia sp.
Perithecia solitaria, superficialia, pyriformia, (128-)139-190(-205) x (115-)122-180(-185) µm, sicca rubra; in aqua vel potassii hydroxidi solutione humectata rubra, in acidis lactico, lutescens; apice acuto. Asci clavati, (35-)38-43(-45) x 4.5-6.0(-8.0) µm, unitunicati, octospori, spice ab annulo, refractili cincto. Ascosporae biseriatae, ellipticae, (7 .0-)7.8-9.3 (-10.0) x 1.9-2.7(-3.0) µm, aequaliter bicellulares, leves, hyalinae. In folibus Collospermi hastati crescens. Holotypus: PDD 44237, Isotypus; NY.
Status anamorphosus: Chaetopsina cf. fulva Rambelli, Atti Accad. Sci. Bologna 15: 5.1956.
NOTES. Conidiophores formed in culture paralleled those found nature except that in nature phialides were strictly terminal whereas culture they were either terminal or intercalary. Cultures were made from ascospores isolated from both of the collections cited above; unfortunately these cultures have been lost. Dried cultures are deposited with the specimens deposited in PDD.
HOLOTYPE. NEW ZEALAND: Gisborne, Urewera National Park, Lake Waikaremoana, vic. park headquarters, track to Lake Ruapani, on decaying leaf of Collospermum hastatum, G.J. Samuels (83-105); P.R. Johnston, T. Matsushima & A.Y. Rossman, 31 May 1983 (PDD 44237; ISOTYPE: NY).
ADDITIONAL SPECIMENS EXAMINED. ECUADOR: Prov. Pichincha, 24 km above Toachi on the Toachi-Palo Quemado Rd., elev. ca. 3400 ft., on twig, K.P. Dumont (Dumont-EC 609), S.E. Carpenter, P. Buritica, 19 Jul 1975 (NY, PDD 44233). JAMAICA: St. Andrew's Parish along Wag Water River, near source, Holywell Recreation area, elev. 2800-3000', on bark, R.P. Korf, J.R. Dixon, K P. Dumont, R .W. Erb, D.H. Pfister, D.R. Reynolds, A.Y. Rossman & G.J. Samuels, 11 Jan 1971 (NY: CUP-MJ 797). NEW ZEALAND: Waitemata City, Waitakere Ranges, Piha Rd., Marguerite Track, on sheathing base of Rhopalostylis sapida G.J. Samuels (80-54) & B. Kendrick, 14 Mar 1980 (PDD 44234, PDD Culture Collection 7802).
Conidiophores scattered, mononematous, erect, setose, unbranched, septate and appearing segmented; red-brown, becoming yellow in 100% lactic acid; walls 2-3 µm thick, 350-370 µm long, tapering from 15-25 µm wide base to ca. 10 µm wide at tip, bearing a single, terminal, colorless, slimy head of conidia on a ca. 25 µm wide penicillus of phialides. Penicillus biseriate, 2-4 primary metulae arising from tip of conidiophore; primary metulae, cylindrical, 10-15 µm long x ca. 4 µm wide, each bearing 2-5 secondary metulae at its tip; secondary metulae cylindrical, 8-10 µm long x ca. 4 µm wide, each bearing ca. 4 phialides. Phialides cylindrical to ampulliform, 10-15 x 3-4 µm wide, with pronounced periclinal thickening at the tip and a flared collarette. Metulae and phialides thin-walled and non-pigmented. Conidia elliptic to subfusoid, tips rounded to subacute, base rounded with a flat, protuberant abscission scar, (8.0-)9.4-22.9(-26.0) x (5.0-)5.9-10.3(-11.0) µm, unicellular, hyaline. Sterile setae present, similar to conidiophores. Perithecia solitary, scattered, superficial, seated on an inconspicuous, non-erumpent basal stroma; red or red above and yellow below, becoming uniformly red in 3% KOH and yellow in 100% lactic acid, pyriform with an acute apex, smooth, not collapsing when dry, (260-)288-345 µm high x (220-)228-260 µm wide. Cells at surface of perithecial wall textura epidermoidea, adjacent cells joined by a pore, walls 1.5-2.0 µm thick. Perithecial wall ca. 15 µm wide, cells at surface, when seen in section, with flat to circular, 2-5 µm wide lumens, walls 1.5-2.0 µm thick, cells becoming progressively more flattened and thin-walled toward the perithecial locule; perithecial apex comprising palisade of parallel, hyphal elements with narrow lumens and rounded tips, walls 1-2 µm thick, elements becoming progressively thinner and more thin-walled toward the ostiolar canal, merging with periphyses. Asci clavate, (80-)93-114(-120) x (14-)18-22(-23) µm, apex simple, 3-8-spored, sometimes with 4 normal spores and 4 aborted spores, ascospores biseriate, completely filling each ascus. Ascospores fusiform, (19.0-)24.8-41.9(-48.0) x (5.5-)5.8-9.8(-11.0) µm, equally 2-celled, not constricted at the septum, with ridge like striations, hyaline.
CHARACTERISTICS IN CULTURE. CMD: 1.5 cm diam., flat, transparent, scant, aerial mycelium white, colony white to pale Sienna (Rayner 8), reverse concolorous, some brown pigment spreading into the medium. PDA: 1 cm diam, flat; scant, aerial mycelium scant, white, surface of colony Sienna (Rayner 8), reverse concolorous, Sienna pigment spreading into the medium. OA: 1 cm diam, as PDA but pigmentation less intense, pale red-brown pigment spreading through the medium. Conidial production abundant on all media. Conidiophores scattered, mononematous, unbranched, stiff, erect, red-brown, 150-230 µm long with base ca.10 µm wide, wall ca. 2 µ thick; a single, compact penicillus formed at the tip of each conidiophore. Penicillus biseriate, ca 4 primary metulae arising from the tip of each conidiophore; primary metulae cylindrical, 10-15 µm long x ca. 5 µm wide, each bearing 2-3 secondary metulae or 2-4 phialides; secondary metulae cylindrical, 10-15 long x ca. 5 µm wide, each bearing 2-4 phialides. Phialides cylindrical, 12-16 x 4-5 µm, with periclinal thickening at the tip and a flared, sometimes funnel-shaped collarette. Phialides also arising from vegetative hyphae, 12-30 µm long x 2-4 µm wide, slight periclinal thickening at tip; collarette flared, often cupulate. Conidia + elliptical with apex round or acute and papillate, base round or with a protuberant, flat basal abscission scar, (13.0-)17.6-22.3(-25.0) x (6.0-)6.8-8.2 µm, unicellular, smooth, hyaline, produced in basipetal succession and held in a colorless drop of slime at the tip of each conidiophore and forming a continuous layer of white slime on surface of colony.
KNOWN DISTRIBUTION. Ecuador, Jamaica, New Zealand.
HABITAT. Bark of Beilschmiedia tawa (A. Cunn.) Kirk and sheathing base of palm frond ( Rhopalostylis sapida Wendl. & Drude).

Nectria chaetopsinae similis, sed perithecia (266-)288-345 x (220-)228-260 µm; asci (80-)93-114(-120) x (14-)18-22(-23) µm, apice simplices; ascosporae fusiformes, (19-)24.8-41.9 (-48.0) x (5.5-)5.8-9.8 (-11.0) µm, crasse striatae. In corticibus Beilschmiedia tawa. Holotypus. PDD 44235. Status anamorphosis. Chaetopsina penicillata Samuels sp. nov.

Setae singularitim dispersae, erectae, rectae, septatae, laeves, crasse tunicatae; rubro brunneae, in acidis lactico lutescens, 350-370 µm longae, superne ca. 10 µm crassae, basi 15-25 µm crassi. Phialides e apice gerentes, in penicilli ordinati. Penicilli biverticillati, 1º metulae 10-15 x 4 µm, 2º metulae 8-10 ca. 4 µm; phialides cylindricae vel ampulliformes, 10-15 x 3-4 µm. Conidia ellipticae vel subfusiformes, (8.0-)9.4-22.9(-26.0) x (5.0-)5.9-10.3 (-11.0) µm. In ramunculo. Holotypus. Dumont-EC 609: NY. Status teleomorphosus. Nectria chaetopsinae-penicillatae Samuels

HOLOTYPE. NEW ZEALAND: Auckland, Waitemata City, Waitakere Ranges Piha Rd., Marguerite Track, on bark of Beilschmiedia tawa, G.J Samuels (77 -21), 21 Mar 1977 (PDD 44235, PDD Culture Collection 7801, ATCC 56205).

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